Artículos BIOINDhttp://hdl.handle.net/10259/42452024-03-29T07:55:24Z2024-03-29T07:55:24ZOnion (Allium cepa L.) Skin Waste Valorization: Unveiling the Phenolic Profile and Biological Potential for the Creation of Bioactive Agents through Subcritical Water ExtractionTrigueros Andrés, EsterBenito Román, OscarOliveira, Andreia P.Videira, Romeu A.Andrade, Paula B.Sanz Díez, Mª TeresaBeltrán Calvo, Sagrariohttp://hdl.handle.net/10259/87782024-03-09T01:05:26Z2024-02-01T00:00:00ZOnion (Allium cepa L.) Skin Waste Valorization: Unveiling the Phenolic Profile and Biological Potential for the Creation of Bioactive Agents through Subcritical Water Extraction
Trigueros Andrés, Ester; Benito Román, Oscar; Oliveira, Andreia P.; Videira, Romeu A.; Andrade, Paula B.; Sanz Díez, Mª Teresa; Beltrán Calvo, Sagrario
Onion skin waste (OSW), the primary non-edible byproduct from onion processing, offers a renewable source of bioactive compounds. This study aims to valorize OSW through subcritical water extraction (SWE), aligning with a circular economy and biorefinery principles. SWE was carried out at 145 °C and 50 bar for 50 min in a discontinuous reactor, producing a phenolic-rich extract (32.3 ± 2.6 mg/g) dominated by protocatechuic acid (20.3 ± 2.5 mg/g), quercetin-4′-O-glucoside (7.5 ± 0.2 mg/g), and quercetin (3.2 ± 0.6 mg/g). Additionally, the extract contains sugars (207.1 ± 20.3 mg sucrose-Eq/g), proteins (22.8 ± 1.6 mg BSA-Eq/g), and free amino acids (20.4 ± 1.2 mg arginine-Eq/g). Its phenolic richness determines its scavenging activity against ●NO and O2●− radicals and its α-glucosidase and aldose-reductase inhibition without affecting α-amylase. Notably, the extract demonstrates significant α-glucosidase inhibition (IC50 = 75.6 ± 43.5 µg/mL), surpassing acarbose (IC50 = 129.5 ± 1.0 µg/mL) in both pure enzyme and cell culture tests without showing cytotoxicity to AGS, HepG2, and Caco-2 human cell lines. The extract’s bioactivity and nutritional content make it suitable for developing antioxidant and antidiabetic nutraceutical/food components, highlighting SWE’s potential for OSW valorization without using organic solvents.
2024-02-01T00:00:00ZImmobilization of naringinase on asymmetric organic membranes: Application for debittering of grapefruit juiceGonzález Temiño, YaizaRuiz Pérez, María OlgaOrtega Santamaría, NatividadRamos Gómez, SoniaBusto Núñez, Mª Doloreshttp://hdl.handle.net/10259/85512024-02-02T01:05:25Z2021-10-01T00:00:00ZImmobilization of naringinase on asymmetric organic membranes: Application for debittering of grapefruit juice
González Temiño, Yaiza; Ruiz Pérez, María Olga; Ortega Santamaría, Natividad; Ramos Gómez, Sonia; Busto Núñez, Mª Dolores
An enzymatic membrane reactor (EMR) is assembled for the immobilization of naringinase on a polyethersulfone ultrafiltration membrane, based on fouling-induced method. The effects of molecular weight cut-off, membrane configuration, applied pressure, enzyme concentration and pH are studied in terms of permeate rate, immobilization efficiency, and biocatalytic conversion. The 10 kDa membrane operating in reverse mode, 0.2 MPa, 0.3 gL−1 of enzyme in acetate buffer at pH 5 and cross-linking with 0.25% glutaraldehyde showed the highest naringin conversion (73%). It was determined that the intermediate pore blocking model was the predominant fouling mechanism for the enzymatic immobilization. The EMR was applied for debittering of grapefruit juice, achieving a conversion of naringin below bitterness threshold and maintaining the antioxidant capacity of the juice. Furthermore, the biocatalytic activity of immobilized enzyme was retained at a high level at least during three consecutive reaction runs, with overnight storage at 4 °C after each run.
2021-10-01T00:00:00ZComparison of external and submerged membranes used in anaerobic membrane bioreactors: Fouling related issues and biological activityMartínez Díaz, RaquelRuiz Pérez, María OlgaRamos Rodríguez, CiprianoCámara Nebreda, José MaríaDiez Blanco, Victorinohttp://hdl.handle.net/10259/82782024-01-11T01:05:23Z2020-07-01T00:00:00ZComparison of external and submerged membranes used in anaerobic membrane bioreactors: Fouling related issues and biological activity
Martínez Díaz, Raquel; Ruiz Pérez, María Olga; Ramos Rodríguez, Cipriano; Cámara Nebreda, José María; Diez Blanco, Victorino
Anaerobic sludge filtration tests were performed with two experimental set-ups equipped with either submerged hollow-fiber membranes or external tubular membranes operated with low crossflow velocity and gas sparging in a gas-lift mode. Particle size distributions, extracellular polymeric substance concentrations, methanogenic activity, reversible and irreversible fouling rates, and chemical cleaning efficiency were all studied and compared. An increase in the percentage of smaller particles and a decrease in the concentration of volatile suspended solids in the external membrane set-up were observed. Both soluble protein and soluble polysaccharide concentrations were also higher in the external filtration. Operating at 12–15 L/m2 h for 7 days, the total resistance of the external membrane reached 9.3 × 1012 m−1, while the resistance of submerged membrane reached 1.1 × 1012 m−1 despite operating at 15–25 L/m2 h for 12 days. Finally, the type of fouling and the efficiency of chemical cleaning also differed in both configurations.
2020-07-01T00:00:00ZA novel jet-loop anaerobic filter membrane bioreactor treating raw slaughterhouse wastewater: Biological and filtration processesDiez Blanco, VictorinoCámara Nebreda, José MaríaRuiz Pérez, María OlgaMartínez Díaz, RaquelRamos Rodríguez, Ciprianohttp://hdl.handle.net/10259/82772024-01-11T01:05:22Z2021-03-01T00:00:00ZA novel jet-loop anaerobic filter membrane bioreactor treating raw slaughterhouse wastewater: Biological and filtration processes
Diez Blanco, Victorino; Cámara Nebreda, José María; Ruiz Pérez, María Olga; Martínez Díaz, Raquel; Ramos Rodríguez, Cipriano
Results from raw slaughterhouse wastewater treatment in a new jet-loop Anaerobic Filter Membrane Bioreactor (AnFMBR) are presented. The innovation consists in integrating an attached growth anaerobic reactor and a filtration tank as an external loop gas-lift bioreactor, taking advantage of the gas-lift effect caused by the gas sparging used for fouling mitigation. A jet-loop AnFMBR pilot plant was operated for 18 months with a hydraulic retention time of 19.5–21.3 h, a solid retention time of 60 d and organic loading rates between 3.5 and 7.4 kg COD/m3·d, depending on the wastewater concentration, with peaks of up to 10.9 kg COD/m3·d. Organic matter removal efficiency was maintained between 92 and 97%. Most of the biomass in the jet-loop AnFMBR, 82%, was retained by the carrier material so the membrane was in contact with low concentration biomass suspension.
Effects of different backwash scenarios and filtration fluxes on reversible and irreversible fouling rates were studied by short-term and mid-term assays, using Box-Behnken experimental designs. Fouling consolidation due to the fouling layer compression was the main cause of irreversible fouling. Pilot-scale results have shown that jet-loop AnFMBR is a promising technology for real scale complex wastewater treatment.
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