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Título : Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
Autor : Pino García, Raquel del
García Lomillo, Javier
Rivero Pérez, Maria Dolores
González San José, Mª Luisa
Muñiz Rodríguez, Pilar
Publicado en: Journal of Agricultural and Food Chemistry. 2015, V. 63, n. 31, p. 6835-7094
Editorial : American Chemical Society
Fecha de publicación : ago-2015
ISSN : 0021-8561
Resumen : Evaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and highlight the validity of QUENCHER (Q) methods, values of Q-method validation were showed for the first time, and they were tested with products of well-known different chemical properties. Furthermore, new QUENCHER assays to measure scavenging capacity against superoxide, hydroxyl, and lipid peroxyl radicals were developed. Calibration models showed good linearity (R2 > 0.995), proportionality and precision (CV < 6.5%), and acceptable detection limits (<20.4 nmol Trolox equiv). The presence of ethanol in the reaction medium gave antioxidant capacity values significantly different from those obtained with water. The dilution of samples with powdered cellulose was discouraged because possible interferences with some of the matrices analyzed may take place.
Palabras clave: antioxidant activities
free radical scavenging assays
URI : http://hdl.handle.net/10259/4294
Versión del editor: http://dx.doi.org/10.1021/jf5042678
Aparece en las colecciones: Artículos BBT

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