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Título : A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-L-Ascorbic Acid Trisodium Salt as a Substrate
Autor : Alvarado Gámez, Ana Lorena
Alonso Lomillo, Mª Asunción
Domínguez Renedo, Olga
Arcos Martínez, Julia
Publicado en: Sensors, 2015, V. 15, n. 2, p. 2232-2243
Editorial : MDPI
Fecha de publicación : feb-2015
Resumen : This paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-L-ascorbic acid trisodium salt, used as substrate, was affected by tungsten ions, which resulted in a decrease of chronoamperometric current, when a potential of 200 mV was applied on 10 mM of substrate in a Tris HCl buffer pH 8.00 and 0.36 M of KCl. Calibration curves for the electrochemical method validation, give a reproducibility of 5.2% (n = 3), a repeatability of 9.4% (n = 3) and a detection limit of 0.29 ± 0.01 μM. Enriched tap water, purified laboratory water and bottled drinking water, with a certified tungsten reference solution traceable to NIST, gave a recovery of 97.1%, 99.1% and 99.1% respectively (n = 4 in each case) and a dynamic range from 0.6 to 30 μM. This study was performed by means of a Lineweaver–Burk plot, showing a mixed kinetic inhibition.
Palabras clave: alkaline phosphatase
chronoamperometry
2-phospho-L-ascorbic acid trisodium salt
screen printed carbon electrode
tungsten
URI : http://hdl.handle.net/10259/4379
Versión del editor: http://dx.doi.org/10.3390/s150202232
Aparece en las colecciones: Artículos ELAN

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