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Título : A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination
Autor : Alvarado Gámez, Ana Lorena
Alonso Lomillo, Mª Asunción
Domínguez Renedo, Olga
Arcos Martínez, Julia
Publicado en: Sensors, 2014, V. 14, n. 2, p. 3756-3767
Editorial : MDPI
Fecha de publicación : feb-2014
Resumen : A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water
Palabras clave: alkaline phosphatase
biosensor
gold nanoparticles
4-nitrophenyl phosphate
screen printed electrode
vanadium
water analysis
Licencia: http://creativecommons.org/licenses/by/3.0/
URI : http://hdl.handle.net/10259/4381
Versión del editor: http://dx.doi.org/10.3390/s140203756
Aparece en las colecciones: Artículos ELAN

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