Universidad de Burgos Repositorio Repositorio
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10259/4724

Ver estadísticas de uso
Título : Comparing ancient DNA survival and proteome content in 69 archaeological cattle tooth and bone samples from multiple European sites
Autor : Wadsworth, Caroline .
Procopio, Noemi .
Anderung, Cecilia .
Carretero, José Miguel
Iriarte Avilés, Eneko
Valdiosera, Cristina .
Rengert Elburg .
Kirsty Penkman .
Buckley, Michael
Publicado en: Journal of Proteomics.2017, V. 158, p. 1-8
Editorial : Elsevier
Fecha de publicación : mar-2017
ISSN : 1874-3919
DOI: 10.1016/j.jprot.2017.01.004
Resumen : Ancient DNA (aDNA) is themost informative biomolecule extracted fromskeletal remains at archaeological sites, but its survival is unpredictable and its extraction and analysis is time consuming, expensive and often fails. Several proposed methods for better understanding aDNA survival are based upon the characterisation of some aspect of protein survival, but these are typically non-specific; proteomic analyses may offer an attractive method for understanding preservation processes. In this study, in-depth proteomic (LC-Orbitrap-MS/MS) analyseswere carried out on 69 archaeological bovine bone and dentine samples from multiple European archaeological sites and comparedwith mitochondrial aDNA and amino acid racemisation (AAR) data. Comparisons of these data, including estimations of the relative abundances for seven selected non-collagenous proteins, indicate that the survival of aDNA in bone or dentine may correlatewith the survival of some proteins, and that proteome complexity is a more useful predictor of aDNA survival than protein abundance or AAR. The lack of a strong correlation between the recovery of aDNA and the proteome abundance may indicate that the survival of aDNA is more closely linked to its ability to associate with bone hydroxyapatite crystals rather than to associate with proteins. Significance: Ancient biomolecule survival remains poorly understood, even with great advancements in ‘omics’ technologies, both in genomics and proteomics. This study investigates the survival of ancient DNA in relation to that of proteins, taking into account proteome complexity and the relative protein abundances to improve our understanding of survival mechanisms. The results show that although protein abundance is not necessarily directly related to aDNA survival, proteome complexity appears to be.
Palabras clave: Ancient DNA
Ancient proteins
Non-collagenous proteins
Licencia: https://creativecommons.org/licenses/by-nc-nd/4.0/
URI : http://hdl.handle.net/10259/4724
Versión del editor: https://doi.org/10.1016/j.jprot.2017.01.004
Aparece en las colecciones: Artículos LEH

Ficheros en este ítem:

Fichero Descripción Tamaño Formato
Wadsworth-JP_2017.pdf909,34 kBAdobe PDFVisualizar/Abrir

Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons
Creative Commons

Los ítems del Repositorio Institucional de la Universidad de Burgos están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2008 MIT and Hewlett-Packard - Sobre DSpace