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Título : Potassium‐ion‐selective fluorescent sensors to detect cereulide, the emetic toxin of B. cereus, in food samples and HeLa cells
Autor : García Calvo, José
Ibeas Cortes, Saturnino
Antón García, Eva Clara
Torroba Pérez, Tomás
González Aguilar, Gerardo .
Antunes, Wilson .
González Lavado, Eloísa .
Fanarraga, Mónica L. .
Publicado en: Chemistry Open. 2017, V. 6, n. 4, p. 562-570
Editorial : Wiley-VCH Verlag
Fecha de publicación : ago-2017
ISSN : 2191-1363
DOI: 10.1002/open.201700057
Resumen : We report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of Bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. For this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. The fluorescence detection of natural cereulide in rice samples was achieved by using synthetic cereulide as a reference and a potassium fluorescent reporter, and this was found to be useful as a portable and fast method for the in situ detection of cereulide in food extracts. To study the fate of cereulide in live cells, we designed a procedure that was suitable for live‐cell microscopy imaging of HeLa cells by comparing the cellular location of the potassium fluorogenic probe, which stained intracellular endolysosomes, in the absence and presence of cereulide; we concluded that in the presence of cereulide, the fluorescence of the probe was decreased because of complexation of the potassium ions by cereulide.
URI : http://hdl.handle.net/10259/4776
Versión del editor: https://doi.org/10.1002/open.201700057
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