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Título : Pectin methylesterase inactivation by High Pressure Carbon Dioxide (HPCD)
Autor : Benito Román, Oscar
Sanz Díez, Mª Teresa
Illera Gigante, Alba Ester
Melgosa Gómez, Rodrigo
Benito Moreno, José Manuel
Beltrán Calvo, Sagrario
Publicado en: The Journal of Supercritical Fluids. 2019, V. 145, p. 111-121
Editorial : Elsevier
Fecha de publicación : mar-2019
Fecha de disponibilidad: 1-abr-2021
ISSN : 0896-8446
DOI: 10.1016/j.supflu.2018.11.009
Resumen : In the present work the effect of High Pressure Carbon Dioxide (HPCD) on the activity and structure of a commercial pectin methylesterase (PME) was studied and the results were compared to the conventional thermal inactivation technology. The use of supercritical CO2 (pressure 6–18 MPa, temperature 40–55 °C and time up to 75 min) increased dramatically the PME inactivation rate. The experimental data fitted the first order model and the inactivation kinetic study of PME was completed with the calculations of the activation energy and volume of activation. HPCD treatment induced important changes in the tertiary structure of the enzyme, as revealed the intrinsic fluorescence, KI quenching and ANS binding analyses. They showed that HPCD treatment rearranged the structure of the enzyme. These changes induced by HPCD were significantly different from those induced by mild thermal treatment. Finally, the structural changes detected correlated with the enzyme activity losses observed.
Palabras clave: PME
HPCD
Enzyme inactivation
CO2
Juice
Materia: Ingeniería química
Chemical engineering
Licencia: https://creativecommons.org/licenses/by-nc-nd/4.0/
URI : http://hdl.handle.net/10259/5045
Versión del editor: https://doi.org/10.1016/j.supflu.2018.11.009
Aparece en las colecciones: Artículos BIOIND

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