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Título : Studies of polyphenol oxidase inactivation by means of high pressure carbon dioxide (HPCD)
Autor : Benito Román, Oscar
Sanz Díez, Mª Teresa
Melgosa Gómez, Rodrigo
Paz Barragán, Esther de
Escudero Barbero, Isabel
Beltrán Calvo, Sagrario
Publicado en: The Journal of Supercritical Fluids. 2019, V. 147, p. 310-321
Editorial : Elsevier
Fecha de publicación : may-2019
Fecha de disponibilidad: jun-2021
ISSN : 0896-8446
DOI: 10.1016/j.supflu.2018.07.026
Resumen : Tyrosinase from mushroom was used as a model polyphenol oxidase (PPO) enzyme to perform a systematic inactivation study using High Pressure Carbon Dioxide (HPCD). The ratio CO2/volume of enzyme (g/mL) loaded in the reactor was found to be critical. Above a critical ratio, pressure, temperature and time did not control the inactivation performance. Exposure time (2–15 min), temperature (25–45 °C) and pressure (5–20 MPa) allowed to show a characteristic inactivation pattern for PPO: a sudden decrease in activity (at least 75% of the total activity loss was observed within the first 2 min) was followed by a slowed decay. The experimental data were fitted into a two fraction kinetic model and the main kinetic parameters (ZP, ZT activation volume and activation energy) were calculated. The fluorescence spectroscopy analysis of the samples treated with HPCD revealed significant changes in the tertiary structure of the enzyme.
Palabras clave: Polyphenol oxidase
HPCD
Enzyme inactivation
Supercritical carbon dioxide
Materia: Ingeniería química
Chemical engineering
Licencia: https://creativecommons.org/licenses/by-nc-nd/4.0/
URI : http://hdl.handle.net/10259/5076
Versión del editor: https://doi.org/10.1016/j.supflu.2018.07.026
Aparece en las colecciones: Artículos BIOIND

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