RT info:eu-repo/semantics/article
T1 Film-shaped reusable smart polymer to produce lactose-free milk by simple immersion
A1 Vallejo García, Jorge Lucas
A1 Arnáiz Alonso, Ana
A1 Busto Núñez, Mª Dolores
A1 García Pérez, José Miguel
A1 Vallejos Calzada, Saúl
K1 β-galactosidase
K1 Lactose-free
K1 Low-lactose
K1 Lactose hydrolysis
K1 Enzyme inmobilization
K1 Química orgánica
K1 Chemistry, Organic
K1 Bioquímica
K1 Biochemistry
K1 Alimentos
K1 Food
AB In this study, we report the synthesis and characterization of a highly manageable polyacrylic film material for enzyme immobilization, using β-galactosidase (β-gal) as a model enzyme. The material is based on commercially available monomers and achieves efficient immobilization of β-gal through the formation of azo linkages between amino styrene groups in the polyacrylic material and the enzyme. The immobilized enzyme demonstrates superior performance compared to free enzyme in lactose hydrolysis of UHT milk, achieving lactose concentrations below 0.1% (<1 mg/mL), indicating its potential for lactose hydrolysis in dairy products. The film-shaped material is designed for easy submersion and removal, similar to a smart card, and offers reusability, with the ability to be reused at least 10 times without loss of enzymatic activity. Characterization of the immobilized enzyme on the polymeric material was performed using various techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Protein release studies confirmed the stability of the immobilized enzyme during prolonged incubation in aqueous solution without significant enzyme leakage. Overall, the polyacrylic film material demonstrates promise as a simple and efficient approach for enzyme immobilization, with potential applications in various industries, including the food industry.
PB Elsevier
SN 0014-3057
YR 2023
FD 2023-11
LK http://hdl.handle.net/10259/7922
UL http://hdl.handle.net/10259/7922
LA eng
NO We gratefully acknowledge the financial support provided by all funders. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author J. M. García received grant PID2020-113264RB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. A. Arnaiz received funding from Ministerio de Universidades-European Union in the frame of NextGenerationEU RD 289/2021 (Universidad Politécnica de Madrid). J. L. Vallejo-García received the grant PRE2021-09812 funded by MCIN/AEI/ 10.13039/501100011033 and by “ESF Investing in your future”. Author Saul Vallejos received grant BG22/00086 funded by Spanish Ministerio de Universidades.
DS Repositorio Institucional de la Universidad de Burgos
RD 09-may-2024