https://hdl.handle.net/10259/11210 2026-05-14T18:29:24Z 2026-05-14T18:29:24Z Ramos-Hernández, Rafael Mielgo Ayuso, Juan Fernández-Lázaro, Diego Abia, Alba Pérez-López, Juan F. Saiz Rodríguez, Miriam Busto Vázquez, Natalia https://hdl.handle.net/10259/11621 2026-05-14T08:16:13Z 2026-02-01T00:00:00Z

Creatine plus β-Hydroxy-β-Methylbutyrate supplementation is associated with preserved glutathione redox-balance and redox–function associations in older adults: a secondary analysis of a randomized crossover trial Ramos-Hernández, Rafael; Mielgo Ayuso, Juan; Fernández-Lázaro, Diego; Abia, Alba; Pérez-López, Juan F.; Saiz Rodríguez, Miriam; Busto Vázquez, Natalia Oxidative stress contributes to age-related musculoskeletal decline, partly through disruption of glutathione-dependent redox homeostasis. Although creatine and β-hydroxy-β-methylbutyrate (HMB) have been individually linked to antioxidant and cytoprotective effects, their combined influence on systemic redox balance in older adults remains insufficiently characterized.To examine the effects of creatine plus HMB supplementation on oxidative stress biomarkers and composite redox indices, and to explore whether redox adaptations co-vary with changes in functional measures in physically active older adults.In a randomized, double-blind, placebo-controlled crossover trial, 30 physically active older adults (62.7 ± 5.3 years; 20 men, 10 women) completed two 6-week intervention phases (3 g/day creatine + 3 g/day calcium HMB vs. placebo) during supervised exercise training. Primary endpoints were oxidized glutathione and the Glutathione Redox Index. Secondary biomarkers and composite indices were analyzed with false discovery rate (FDR) control. Percent changes (Δ%) in functional tests were examined exclusively as exploratory correlates of redox adaptations.Supplementation was associated with attenuation of the placebo-related increase in oxidized glutathione and nominal preservation of the Glutathione Redox Index, although these effects did not remain significant after FDR adjustment. In men, a nominal increase in malondialdehyde was observed under supplementation. Exploratory analyses indicated weak associations between changes in composite redox indices and Δ% functional measures.Creatine plus HMB supplementation was associated with nominal modulation of glutathione-centered redox balance during training in active older adults. Exploratory redox–function associations support further investigation in larger, adequately powered trials.

2026-02-01T00:00:00Z Pérez Gómez, Noelia Martínez-Zamora, María Ángeles Carmona, Francisco Novalbos, Jesús Abad-Santos, Francisco Koller, Dora Saiz Rodríguez, Miriam https://hdl.handle.net/10259/11620 2026-05-14T07:57:44Z 2026-03-01T00:00:00Z

An Overview of Endometriosis and Potential Pharmacogenetic Targets Pérez Gómez, Noelia; Martínez-Zamora, María Ángeles; Carmona, Francisco; Novalbos, Jesús; Abad-Santos, Francisco; Koller, Dora; Saiz Rodríguez, Miriam Endometriosis is a chronic inflammatory disease characterized by the presence of endometrial-like tissue outside the uterine cavity. It affects approximately 10–15% of women of reproductive age globally and is characterized by heterogeneous symptoms with chronic pelvic pain, dysmenorrhea, and infertility being the most common. Although pharmacological treatments are available to manage its symptoms, many women either do not respond to these therapies or experience adverse drug reactions (ADRs) that outweigh the original symptoms of endometriosis. Current research highlights a critical gap in pharmacogenetic biomarkers for endometriosis treatment, limiting the potential for personalized therapeutic strategies. Integrative multi-omics approaches combining genetic, inflammatory, and hormonal profiles may enhance patient stratification and optimize individualized care.

2026-03-01T00:00:00Z Ramos-Hernández, Rafael Busto Vázquez, Natalia Miguel Ortega, Alvaro Martínez Ferrán, María Santamaría Peláez, Mirian Saiz Rodríguez, Miriam Mielgo Ayuso, Juan https://hdl.handle.net/10259/11612 2026-05-14T06:37:58Z 2026-01-01T00:00:00Z

Combined creatine and β-hydroxy-β-methylbutyrate supplementation with integral conditioning exercise enhances functional performance and metabolic health in physically active older adults: A randomized controlled crossover trial Ramos-Hernández, Rafael; Busto Vázquez, Natalia; Miguel Ortega, Alvaro; Martínez Ferrán, María; Santamaría Peláez, Mirian; Saiz Rodríguez, Miriam; Mielgo Ayuso, Juan Objective: to investigate the effects of six weeks of CRE + HMB supplementation integrated with a supervised multicomponent exercise program on functional performance, metabolic efficiency, and physiological health in older adults. Conclusions: six weeks of CRE + HMB supplementation integrated with supervised multicomponent training enhanced mobility, metabolic efficiency, and selected physiological outcomes in physically active older adults. This strategy represents a safe, feasible, and practical approach to sustain functional independence and metabolic health with aging.

2026-01-01T00:00:00Z Busto Vázquez, Natalia Vigueras, Gloria Cutillas, Natalia García Ruiz, Begoña Ruiz, José https://hdl.handle.net/10259/11392 2026-02-19T01:05:24Z 2022-07-01T00:00:00Z

Inert cationic iridium(iii) complexes with phenanthroline-based ligands: application in antimicrobial inactivation of multidrug-resistant bacterial strains Busto Vázquez, Natalia; Vigueras, Gloria; Cutillas, Natalia; García Ruiz, Begoña; Ruiz, José The antimicrobial activity of a new series of heteroleptic iridium(III) complexes of the type [Ir(C^N)2(N^N)][PF6] (C^N = deprotonated 2-phenylbenzimidazole-κN, κC; N^N = phen (Ir1), dpq (Ir2), dppz (Ir3), dppn (Ir4), and dppz-idzo (Ir5)) was studied towards two Gram positive (vancomycin-resistant Enterococcus faecium and a methicillin-resistant Staphylococcus aureus) and two Gram negative (Acinetobacter baumanii and Pseudomonas aeruginosa) multidrug-resistant bacterial strains of clinical interest. Although the complexes were inactive towards Gram negative bacteria, their effectiveness against Gram positive strains was remarkable, especially for Ir1 and Ir2, the most bactericidal complexes that were even more active (10 times) than the fluoroquinolone antibiotic norfloxacin and displayed no toxicity in human kidney cells (HEK293). Mechanistic studies revealed that the cell wall and membrane of MRSA S. aureus remained intact on treatment with these compounds and that DNA was not their main target. It is important to note that the complexes were able to induce ROS generation, this being the feature key to their antimicrobial activity.

2022-07-01T00:00:00Z