View Item 

Show simple item record

dc.contributor.authorMartínez Alonso, Marta 
dc.contributor.authorGandioso, Albert
dc.contributor.authorThibaudeau, Chloé
dc.contributor.authorQin, Xue
dc.contributor.authorArnoux, Philippe
dc.contributor.authorDemeubayeva, Nurikamal
dc.contributor.authorGuérineau, Vincent
dc.contributor.authorFrochot, Céline
dc.contributor.authorJung, Alain C.
dc.contributor.authorGaiddon, Christian
dc.contributor.authorGasser, Gilles
dc.date.accessioned2026-02-04T13:52:34Z
dc.date.available2026-02-04T13:52:34Z
dc.date.issued2023-08
dc.identifier.issn1439-4227
dc.identifier.urihttps://hdl.handle.net/10259/11326
dc.description.abstractA novel Ru(II) cyclometalated photosensitizer (PS), Ru-NH2, for photodynamic therapy (PDT) of formula [Ru(appy)(bphen)2]PF6 (where appy=4-amino-2-phenylpyridine and bphen=bathophenanthroline) and its cetuximab (CTX) bioconjugates, Ru-Mal-CTX and Ru-BAA-CTX (where Mal=maleimide and BAA=benzoylacrylic acid) were synthesised and characterised. The photophysical properties of Ru-NH2 revealed absorption maxima around 580 nm with an absorption up to 725 nm. The generation of singlet oxygen (1O2) upon light irradiation was confirmed with a 1O2 quantum yield of 0.19 in acetonitrile. Preliminary in vitro experiments revealed the Ru-NH2 was nontoxic in the dark in CT-26 and SQ20B cell lines but showed outstanding phototoxicity when irradiated, reaching interesting phototoxicity indexes (PI) >370 at 670 nm, and >150 at 740 nm for CT-26 cells and >50 with NIR light in SQ20B cells. The antibody CTX was successfully attached to the complexes in view of the selective delivery of the PS to cancer cells. Up to four ruthenium fragments were anchored to the antibody (Ab), as confirmed by MALDI-TOF mass spectrometry. Nonetheless, the bioconjugates were not as photoactive as the Ru-NH2 complex.en
dc.description.sponsorshipThe European Commission H2020-MSCA-IF-2018 grant supportedthis work, OrganometRuPDT (839499) to M.M. This work wasfinancially supported by an ERC Consolidator Grant PhotoMedMetto G.G. (GA 681679) and has received support under the program‘‘Investissements d’Avenir’’ launched by the French Governmentand implemented by the ANR with the reference ANR-10-IDEX-0001-02 PSL (G.G.), by the ITMO Cancer AVIESAN (AllianceNationale pour les Sciences de la Vie et de la Sante/ NationalAlliance for Life Sciences & Health) within the framework of theCancer Plan (G.G, C.G and A.J.). A.G thanks the ARC Foundationfor cancer research for a postdoctoral research fellowship. TheStreinth Team is supported by the Association pour la Recherchesur le Cancer, European action COST Proteocure, the Interdiscipli-nary thematic Institute InnoVec, the IDEX Excellence grant fromUnistra, Itmo Cancer, and the Institut National du Cancer. C.T. isfinancially supported by a PhD fellowship awarded by theDoctoral School ED414 of the University of Strasbourg.en
dc.format.mimetypeapplication/pdf
dc.language.isoenges
dc.publisherWileyes
dc.relation.ispartofChemBioChem. 2023, V. 24, n. 15, e202300203es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAntibodyen
dc.subjectBioconjugationen
dc.subjectCetuximaben
dc.subjectMetals inmedicineen
dc.subjectPhotodynamic therapyen
dc.subjectRutheniumen
dc.subject.otherFotoquimioterapiaes
dc.subject.otherPhotochemotherapyen
dc.subject.otherCáncer-Tratamientoes
dc.subject.otherCancer-Treatmenten
dc.titleA Novel Near‐IR Absorbing Ruthenium(II) Complex as Photosensitizer for Photodynamic Therapy and its Cetuximab Bioconjugatesen
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttps://doi.org/10.1002/cbic.202300203es
dc.identifier.doi10.1002/cbic.202300203
dc.identifier.essn1439-7633
dc.journal.titleChemBioChemes
dc.volume.number24es
dc.issue.number15es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


Files in this item

Thumbnail
Name:
Martinez-ChemBioChem_2023.pdf
Size:
4.086Mb
Format:
Adobe PDF
FilesOpen

This item appears in the following Collection(s)

Show simple item record