2026-05-08T11:06:24Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/101082025-01-31T01:05:23Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314

Santamaría Fernández, Mª Estrella dacebd9d-9971-4cfc-98c8-07ae48e159cf 600 Arnáiz Alonso, Ana 762 600 0000-0002-3842-498X Velasco Arroyo, Blanca 829 600 0000-0001-5214-6499 Grbic, Vojislava cddc656d-4502-4a51-a2a7-bc7e3901048b 600 Diaz, Isabel d8f3d8e6-ddde-40c2-b67f-eaeddc3c9350 600 Martínez, Manuel fd739134-9cc0-4cd9-9776-904aca07e812 600 2025-01-30T13:10:46Z 2025-01-30T13:10:46Z 2018 http://hdl.handle.net/10259/10108 10.1038/s41598-018-27904-1 2045-2322 Reactive oxygen species (ROS) are molecules that play a prominent role in plant response to numerous stresses, including plant interactions with herbivores. Previous findings indicate that Arabidopsis plants showed an increase in H2O2 accumulation after Tetranychus urticae infestation. Despite its importance, no information has been reported on the relationships between ROS-metabolizing systems and the spider mite-triggered plant-induced responses. In this work, four ROS-related genes that were differentially expressed between the resistant Bla-2 and the susceptible Kon Arabidopsis accessions were selected for the analysis. These genes encode proteins putatively involved in the generation (BBE22) and degradation (GPX7 and GSTU4) of H2O2, and in the degradation of ascorbate (AO). Overexpressing BBE22 and silencing GPX7, GSTU4 and AO resulted in higher leaf damage and better mite performance relative to the wild-type plants. Minor effects on H2O2 accumulation obscure major effects on the expression of genes related to ROS-metabolism and JA and SA signaling pathways, and on ROS-related enzymatic activities. In conclusion, the integration of ROS and ROS-related compounds and enzymes in the response of Arabidopsis to the spider mite T. urticae was confirmed. However, the complex network involved in ROS signaling makes difficult to predict the impact of a specific genetic manipulation. This work was supported by projects BIO2014-53508-R and 618105-FACCE-Era Net Plus from Ministerio de Economía y Competitividad. application/pdf eng Nature Scientific Reports. 2018, V. 8, n. 1, 9432 https://doi.org/10.1038/s41598-018-27904-1 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Herbivory Plant signalling Biotecnología Bioquímica Biotechnology Biochemistry Arabidopsis response to the spider mite Tetranychus urticae depends on the regulation of reactive oxygen species homeostasis info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Scientific Reports 8 1 9432 THUMBNAIL Santamaría-sr_2018.pdf.jpg Santamaría-sr_2018.pdf.jpg IM Thumbnail image/jpeg 5045 https://riubu.ubu.es/bitstream/10259/10108/4/Santamar%c3%ada-sr_2018.pdf.jpg 01081ed6c66410a6c0e409251d72fcaf MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/10108/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/10108/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Santamaría-sr_2018.pdf Santamaría-sr_2018.pdf application/pdf 1926893 https://riubu.ubu.es/bitstream/10259/10108/1/Santamar%c3%ada-sr_2018.pdf c80af41351abd8e4f5fd84802119fa22 MD5 1 10259/10108 oai:riubu.ubu.es:10259/10108 2025-01-31 02:05:23.187 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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