2026-04-18T14:17:25Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/104432025-05-14T10:59:47Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Barea Gómez, Pedro Illera Gigante, Alba Ester Candela Gil, Helena Melgosa Gómez, Rodrigo Benito Moreno, José Manuel Beltrán Calvo, Sagrario Sanz Díez, Mª Teresa Protein hydrolysis Dead-end fltration (UF and NF) Protein and Free amino acids profle Reducing and chelating capacities Molecular weight distribution Two different hydrolysates of the water-soluble protein (WSP) fraction from tuna fish meal were obtained by subcritical water-CO2 (subW-CO2) and Alcalase® treatments. Hydrolysates showed different chemical composition regarding their free amino acid (FAA) profile and molecular weight distribution of the peptides generated. Consequently, different strategies were proposed by using a stirred dead-end filtration system equipped with flat sheet membranes. Two nanofiltration (NF) consecutive steps were proposed for fractionation of subW-CO2 hydrolysates with polyamide membranes (800–600 Da and 300–150 Da) yielding a first retentate where 79% of the protein fraction was retained, while 92% of FAA were retained in the second step with a purity index of 29%. Alcalase® hydrolysates were fractionated by ultrafiltration (UF, 10 kDa) followed by NF (1–1.1 kDa) with polyethersulfone membranes obtaining a first retentate with more than 65% of protein fraction retention with a purity index of 50%. A relatively wide molecular weight distribution was observed in all streams, with high antioxidant and iron-chelating capacities in the retentate fractions. 2025-05-08 2025-05-08 2025-03 info:eu-repo/semantics/article 2197-4365 http://hdl.handle.net/10259/10443 10.1186/s40643-025-00850-3 2197-4365 eng Bioresources and Bioprocessing. 2025, V. 12, n. 16 https://doi.org/10.1186/s40643-025-00850-3 http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional Springer