2026-04-18T16:12:12Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/104432025-05-14T10:59:47Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245
Repositorio Institucional de la Universidad de Burgos
author
Barea Gómez, Pedro
author
Illera Gigante, Alba Ester
author
Candela Gil, Helena
author
Melgosa Gómez, Rodrigo
author
Benito Moreno, José Manuel
author
Beltrán Calvo, Sagrario
author
Sanz Díez, Mª Teresa
2025-05-08T10:50:13Z
2025-05-08T10:50:13Z
2025-03
2197-4365
http://hdl.handle.net/10259/10443
10.1186/s40643-025-00850-3
2197-4365
Two different hydrolysates of the water-soluble protein (WSP) fraction from tuna fish meal were obtained by subcritical water-CO2 (subW-CO2) and Alcalase® treatments. Hydrolysates showed different chemical composition regarding their free amino acid (FAA) profile and molecular weight distribution of the peptides generated. Consequently, different strategies were proposed by using a stirred dead-end filtration system equipped with flat sheet membranes. Two nanofiltration (NF) consecutive steps were proposed for fractionation of subW-CO2 hydrolysates with polyamide membranes (800–600 Da and 300–150 Da) yielding a first retentate where 79% of the protein fraction was retained, while 92% of FAA were retained in the second step with a purity index of 29%. Alcalase® hydrolysates were fractionated by ultrafiltration (UF, 10 kDa) followed by NF (1–1.1 kDa) with polyethersulfone membranes obtaining a first retentate with more than 65% of protein fraction retention with a purity index of 50%. A relatively wide molecular weight distribution was observed in all streams, with high antioxidant and iron-chelating capacities in the retentate fractions.
eng
Atribución 4.0 Internacional
Protein hydrolysis
Dead-end fltration (UF and NF)
Protein and Free amino acids profle
Reducing and chelating capacities
Molecular weight distribution
Membrane fractionation of hydrolysates of the water-soluble protein from tuna fish meal obtained by subcritical water and enzymatic treatments. Comparison of physical and chemical properties
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/10443/1/Barea-b%26b_2025.pdf
File
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57a725808540bb19cc2ceb86dad291e3
2828777
application/pdf
Barea-b&b_2025.pdf