2026-04-18T14:19:39Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/104432025-05-14T10:59:47Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Barea Gómez, Pedro 865 600 0000-0003-0525-327X Illera Gigante, Alba Ester 296 600 0000-0001-8321-5282 Candela Gil, Helena 924 600 0009-0000-8962-4837 Melgosa Gómez, Rodrigo 357 600 0000-0003-2130-2422 Benito Moreno, José Manuel 58 600 0000-0001-8899-0448 Beltrán Calvo, Sagrario 57 600 0000-0003-2559-3925 Sanz Díez, Mª Teresa 530 600 0000-0003-1799-3099 2025-05-08T10:50:13Z 2025-05-08T10:50:13Z 2025-03 2197-4365 http://hdl.handle.net/10259/10443 10.1186/s40643-025-00850-3 2197-4365 Two different hydrolysates of the water-soluble protein (WSP) fraction from tuna fish meal were obtained by subcritical water-CO2 (subW-CO2) and Alcalase® treatments. Hydrolysates showed different chemical composition regarding their free amino acid (FAA) profile and molecular weight distribution of the peptides generated. Consequently, different strategies were proposed by using a stirred dead-end filtration system equipped with flat sheet membranes. Two nanofiltration (NF) consecutive steps were proposed for fractionation of subW-CO2 hydrolysates with polyamide membranes (800–600 Da and 300–150 Da) yielding a first retentate where 79% of the protein fraction was retained, while 92% of FAA were retained in the second step with a purity index of 29%. Alcalase® hydrolysates were fractionated by ultrafiltration (UF, 10 kDa) followed by NF (1–1.1 kDa) with polyethersulfone membranes obtaining a first retentate with more than 65% of protein fraction retention with a purity index of 50%. A relatively wide molecular weight distribution was observed in all streams, with high antioxidant and iron-chelating capacities in the retentate fractions. This work was supported by the Agencia Estatal de Investigación [Grant numbers PID2022-136385OB-I00, PDC2022-133443-I00] and the Junta de Castilla y León (JCyL) and the European Regional Development Fund (ERDF) [Grant number BU027P23]. P. Barea pre-doctoral contract was funded by JCyL and the European Social Fund (ESF) [ORDEN EDU/1868/2022]. A. E. Illera postdoctoral contract was funded by BU027P23. H. Candela-Gil contract was funded by PDC2022-133443-I00. R. Melgosa contract was funded by a Beatriz Galindo Research Fellowship [BG20/00182]. application/pdf eng Springer Bioresources and Bioprocessing. 2025, V. 12, n. 16 https://doi.org/10.1186/s40643-025-00850-3 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Protein hydrolysis Dead-end fltration (UF and NF) Protein and Free amino acids profle Reducing and chelating capacities Molecular weight distribution Ingeniería Química Biotecnología Bioquímica Chemical engineering Biotechnology Biochemistry Membrane fractionation of hydrolysates of the water-soluble protein from tuna fish meal obtained by subcritical water and enzymatic treatments. Comparison of physical and chemical properties info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bioresources and Bioprocessing 12 16 THUMBNAIL Barea-b&b_2025.pdf.jpg Barea-b&b_2025.pdf.jpg IM Thumbnail image/jpeg 4485 https://riubu.ubu.es/bitstream/10259/10443/4/Barea-b%26b_2025.pdf.jpg c98bd506ec5698bd6d28875f1d245fb8 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/10443/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/10443/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Barea-b&b_2025.pdf Barea-b&b_2025.pdf application/pdf 2828777 https://riubu.ubu.es/bitstream/10259/10443/1/Barea-b%26b_2025.pdf 57a725808540bb19cc2ceb86dad291e3 MD5 1 10259/10443 oai:riubu.ubu.es:10259/10443 2025-05-14 12:59:47.435 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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