2026-05-23T20:13:47Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/109282025-10-07T11:19:39Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Arunkumar, Guha Asthagiri 6a0e107e-2718-409f-a305-b32da2eaa783 600 Ioannou, Andriani b5bd6d3b-1a15-44f5-896c-3735c46e8bdd 600 Wohlbold, Teddy John 7f293263-fd83-47c3-a42d-e3d4cf825360 600 Meade, Philip 7eedc109-223d-4ad7-8275-a6a6af85ee40 600 Aslam, Sadaf 34ce451c-1783-43a0-99fe-d911a3c31bcd 600 Amanat, Fatima b8ac8f6c-f358-4a47-8f38-f1af58b43bdb 600 Ayllón Barasoain, Juan 852 600 0000-0002-7556-8106 García Sastre, Adolfo 9de2ea8b-c019-414d-bb90-b1d51ef5bbd7 600 Krammer, Florian 134d72a4-c1a0-45c7-97bf-f4dc99569766 600 2025-10-06T12:02:45Z 2025-10-06T12:02:45Z 2019-03 0022-538X https://hdl.handle.net/10259/10928 10.1128/JVI.01696-18 1098-5514 Protection from influenza virus infection is canonically associated with antibodies that neutralize the virus by blocking the interaction between the viral hemagglutinin and host cell receptors. However, protection can also be conferred by other mechanisms, including antibody-mediated effector functions. Here, we report the characterization of 22 broadly cross-reactive, nonneutralizing antibodies specific for influenza B virus hemagglutinin. The majority of these antibodies recognized influenza B viruses isolated over the period of 73 years and bind the conserved stalk domain of the hemagglutinin. A proportion of the characterized antibodies protected mice from both morbidity and mortality after challenge with a lethal dose of influenza B virus. Activity in an antibody-dependent cell-mediated cytotoxicity reporter assay correlated strongly with protection, suggesting that Fc-dependent effector function determines protective efficacy. The information regarding mechanism of action and epitope location stemming from our characterization of these antibodies will inform the design of urgently needed vaccines that could induce broad protection against influenza B viruses. We thank Ariana Hirsh and Daniel Kaplan for excellent technical support. Andriani Ioannou was supported by an NIAID T32 Virus-Host Interactions training grant (5T32AI007647-17). This work was supported by NIAID grants R01 AI117287, U19 AI109946, and P01 AI097092 and the NIAID Centers of Excellence for Influenza Research and Surveillance (CEIRS) contract HHSN272201400008C. The Krammer and García-Sastre labs are receiving funding for an unrelated influenza virus vaccine project from GlaxoSmithKline. application/pdf eng American Society for Microbiology Journal of Virology. 2019, V. 93, n. 6, e01696-18 https://doi.org/10.1128/JVI.01696-18 Atribución 4.0 Internacional Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess ADCC HA Influenza B MAb Gripe Influenza Broadly Cross-Reactive, Nonneutralizing Antibodies against Influenza B Virus Hemagglutinin Demonstrate Effector Function-Dependent Protection against Lethal Viral Challenge in Mice info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Journal of Virology 93 6 THUMBNAIL Asthagiri-jv_2019.pdf.jpg Asthagiri-jv_2019.pdf.jpg IM Thumbnail image/jpeg 4396 https://riubu.ubu.es/bitstream/10259/10928/4/Asthagiri-jv_2019.pdf.jpg e6de2060f48f2ea3d43f6aefd07afe00 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/10928/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/10928/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Asthagiri-jv_2019.pdf Asthagiri-jv_2019.pdf application/pdf 1601416 https://riubu.ubu.es/bitstream/10259/10928/1/Asthagiri-jv_2019.pdf dc8705e2daa1a32e009a5b97f848cefe MD5 1 10259/10928 oai:riubu.ubu.es:10259/10928 2025-10-07 13:19:39.477 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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