2026-04-28T02:51:29Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/111292025-12-13T01:05:47Zcom_10259_6168com_10259_5086com_10259_2604col_10259_6169

00925njm 22002777a 4500 dc Campillo Cora, Claudia author Soto Gómez, Diego author Arias Estévez, Manuel author Fernández Calviño, David author 2022-09 The assessment of remediation on metal-polluted soils is usually focused on total and/or bioavailable metal content. However, these chemical variables do not provide direct information about reductions in heavy metals pressure on soil microorganisms. We propose the use of bacterial communities to evaluate the efficiency of three remediation techniques: crushed mussel shell (CMS) and pine bark (PB) as soil amendments and EDTA-washing. A soil sample was polluted with different doses of Cu, Ni, and Zn (separately). After 30 days of incubation, the remediation techniques were applied, and bacterial community tolerance to heavy metals determined. If bacterial communities develop tolerance, it is an indicator that the metal is exerting toxicity on them. Soil bacterial communities developed tolerance to Cu, Ni, and Zn in response to metal additions. After remediation, bacterial communities showed decreases in bacterial community tolerance to Cu, Ni, and Zn for all remediation techniques. For Cu and Ni, soil EDTA-washing showed the greatest reduction of bacterial community tolerance to Cu and Ni, respectively, while for Zn the soil amendment with PB was the most effective remediation technique. Thus, bacterial community tolerance to heavy metals successfully detect differences in the effectiveness of the three remediation techniques. 2073-4395 https://hdl.handle.net/10259/11129 10.3390/agronomy12102280 2073-4395 Metal pollution Soil bioremediation Risk assessment Bacterial growth Pollution-Induced Community Tolerance (PICT) Bio-sorbents Soil washing Assessment of Polluted Soil Remediation Using Bacterial Community Tolerance to Heavy Metals as an Indicator