2026-04-28T11:35:20Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/112442026-01-21T01:05:39Zcom_10259_4292com_10259_5086com_10259_2604col_10259_4293

00925njm 22002777a 4500 dc González Angulo, Mario author Clauwers, Charlien author Harastani, Rania author Tonello Samson, Carole author Jaime Moreno, Isabel author Rovira Carballido, Jordi author W. Michiels, Chris author 2020-04 Bacterial spores survive high pressure processing (HPP). Group II Clostridium botulinum is an obligate anaerobe spore-forming pathogen that can produce the botulinum neurotoxin under refrigeration. This study assessed nontoxigenic type E C. botulinum and Group II Clostridium sp. growth in raw and HPP (550 MPa, 3 min, 10 °C) Thai coconut water (CCW; pH 5.2). No spore germination or growth occurred in HPP CCW inoculated with 105 CFU/ml after 61 days regardless of oxygen concentration (<0.5 – 11 mg/l) or storage temperature (4 and 20 °C). Spore concentration decreased by 3.0 ± 0.1 log CFU/ml in a worst-case scenario consisting of non-HPP filter-sterilized CCW (pH 7.0) under anoxic incubation at 30 °C during 61 days, suggesting spore germination followed by cellular death. Supplementing filter-sterilized CCW (pH 7.0) with selected germinants and free amino acids did not support spore development, but the addition of nutrient-rich laboratory media (TPGY broth) at low concentrations (6.25%) promoted growth, suggesting that a lack of nutrients prevents C. botulinum development in CCW. Further risk assessment will require evaluating other CCW varieties and toxin production. 0963-9969 https://hdl.handle.net/10259/11244 10.1016/j.foodres.2020.109278 High pressure processing Coconut water Clostridium botulinum Dissolved oxygen Germinant Amino acid Nutrients Evaluation of factors influencing the growth of non-toxigenic Clostridium botulinum type E and Clostridium sp. in high-pressure processed and conditioned tender coconut water from Thailand