2026-04-28T08:54:57Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/112442026-01-21T01:05:39Zcom_10259_4292com_10259_5086com_10259_2604col_10259_4293

González Angulo, Mario 0a75d6d7-9cbf-4d6e-b17e-49218db21f0b 600 Clauwers, Charlien e76321c3-7b68-4ad9-8785-839952ac468a 600 Harastani, Rania ce46d450-15e5-4b72-8ae0-52bacee23c68 600 Tonello Samson, Carole 4df7e9df-f96e-43af-8daa-5af5e79f4f0d 600 Jaime Moreno, Isabel 301 600 0000-0001-5975-6900 Rovira Carballido, Jordi 490 600 0000-0001-6664-9484 W. Michiels, Chris 35bb4381-8a50-4070-9289-7197e0902300 600 2026-01-20T10:12:31Z 2026-01-20T10:12:31Z 2020-04 0963-9969 https://hdl.handle.net/10259/11244 10.1016/j.foodres.2020.109278 Bacterial spores survive high pressure processing (HPP). Group II Clostridium botulinum is an obligate anaerobe spore-forming pathogen that can produce the botulinum neurotoxin under refrigeration. This study assessed nontoxigenic type E C. botulinum and Group II Clostridium sp. growth in raw and HPP (550 MPa, 3 min, 10 °C) Thai coconut water (CCW; pH 5.2). No spore germination or growth occurred in HPP CCW inoculated with 105 CFU/ml after 61 days regardless of oxygen concentration (<0.5 – 11 mg/l) or storage temperature (4 and 20 °C). Spore concentration decreased by 3.0 ± 0.1 log CFU/ml in a worst-case scenario consisting of non-HPP filter-sterilized CCW (pH 7.0) under anoxic incubation at 30 °C during 61 days, suggesting spore germination followed by cellular death. Supplementing filter-sterilized CCW (pH 7.0) with selected germinants and free amino acids did not support spore development, but the addition of nutrient-rich laboratory media (TPGY broth) at low concentrations (6.25%) promoted growth, suggesting that a lack of nutrients prevents C. botulinum development in CCW. Further risk assessment will require evaluating other CCW varieties and toxin production. application/pdf eng Elsevier Food Research International. 2020, V. 134, p. 109278 https://doi.org/10.1016/j.foodres.2020.109278 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess High pressure processing Coconut water Clostridium botulinum Dissolved oxygen Germinant Amino acid Nutrients Microbiología Microbiología alimentaria Microbiology Food-Microbiology Evaluation of factors influencing the growth of non-toxigenic Clostridium botulinum type E and Clostridium sp. in high-pressure processed and conditioned tender coconut water from Thailand info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Food Research International 134 109278 THUMBNAIL González-fri_2020.pdf.jpg González-fri_2020.pdf.jpg IM Thumbnail image/jpeg 3681 https://riubu.ubu.es/bitstream/10259/11244/4/Gonz%c3%a1lez-fri_2020.pdf.jpg 22bf04b98fbb0be97c222e4ebaadfd93 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/11244/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/11244/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL González-fri_2020.pdf González-fri_2020.pdf application/pdf 949864 https://riubu.ubu.es/bitstream/10259/11244/1/Gonz%c3%a1lez-fri_2020.pdf addb7968639ee953f291ad5d1cea789e MD5 1 10259/11244 oai:riubu.ubu.es:10259/11244 2026-01-21 02:05:39.796 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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