2026-04-28T08:54:52Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/112652026-01-23T01:05:32Zcom_10259_4292com_10259_5086com_10259_2604col_10259_4293

García Sánchez, Lourdes 218 600 0000-0003-3013-3827 Melero Gil, Beatriz 356 600 0000-0001-8982-8186 Diez Mate, Ana Mª 153 600 0000-0003-0176-446X Jaime Moreno, Isabel 301 600 0000-0001-5975-6900 Canepa Oneto, Antonio Jesús 818 600 0000-0002-0608-2743 Rovira Carballido, Jordi 490 600 0000-0001-6664-9484 2026-01-22T11:10:21Z 2026-01-22T11:10:21Z 2020-03 0167-5877 https://hdl.handle.net/10259/11265 10.1016/j.prevetmed.2020.104935 Campylobacter spp. are the leading causes of bacterial human gastroenteritis worldwide; being poultry farms the main source of infections. In order to obtain information on prevalence and diversity of Campylobacter-infected flocks in the North of Spain, fourteen farms were studied between autumn and spring in 2014 and 2015, respectively. Moreover, virulence genes involved in pathogenicity and antimicrobial resistance were investigated. A survey about preventive hygiene practices at farms was performed to determine the risky practices that could contribute to the presence of Campylobacter in this step of the poultry food chain. Testing the presence of Campylobacter spp. showed 43 % of the farms were positive during autumn, whereas only 31 % were positive in spring. A very high prevalence within-flock was observed (43.1 % to 88.6 %) and C. jejuni was the most prevalent species in both periods. Genotyping by pulsed field gel electrophoresis (PFGE) showed a high heterogeneity among farms (309 isolates clustered into 21 pulsotypes). Virulence genes were present in all C. jejuni isolates while cdtA and cdtC were absent in C. coli. On the contrary, the latter showed higher antimicrobial resistance than C. jejuni. This study suggests that environment might be one of the main sources for Campylobacter transmission, as water supply seemed to be a clear cause of the contamination in a specific farm. However, in other farms other environmental factors contributed to the contamination, confirming the multifactorial origin of Campylobacter colonization in broilers. Therefore, biosecurity measures in farms are crucial to reduce Campylobacter contamination, which may have important implications for human and animal health. This work was supported by a project founded by “Ministerio de Economía y Competitividad” from the Spanish Government (AGL 2013-47694-R) and by “Junta de Castilla y Leon” (BU159U14). The PhD grant of Lourdes García Sanchez is funded by University of Burgos, Spain (12-PR00). application/pdf eng Elsevier Preventive Veterinary Medicine. 2020, V. 176, 104935 https://doi.org/10.1016/j.prevetmed.2020.104935 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Poultry farms Prevalence PFGE Virulence genes Antibiotic resistance Spain Salud pública veterinaria Epidemiología Veterinary public health Epidemiology Genotyping, virulence genes and antimicrobial resistance of Campylobacter spp.isolated during two seasonal periods in Spanish poultry farms info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Preventive Veterinary Medicine 176 104935 THUMBNAIL Garcia-pvm_2020.pdf.jpg Garcia-pvm_2020.pdf.jpg IM Thumbnail image/jpeg 3619 https://riubu.ubu.es/bitstream/10259/11265/4/Garcia-pvm_2020.pdf.jpg f3db467603b1bfd660f70bd15b551bda MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/11265/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/11265/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Garcia-pvm_2020.pdf Garcia-pvm_2020.pdf application/pdf 581549 https://riubu.ubu.es/bitstream/10259/11265/1/Garcia-pvm_2020.pdf 95511c3c522b0fc559c650c945b22da0 MD5 1 10259/11265 oai:riubu.ubu.es:10259/11265 2026-01-23 02:05:32.299 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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