2026-05-23T20:13:50Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/113212026-02-05T01:05:55Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Antón Fidalgo, Nuria ee415384-c051-4589-b797-dcc18025a6aa 600 Mendes, Marta V. 205189bc-9aae-42b2-9b18-3552ae187f47 600 Martín, Juan F. 997101d9-25d8-4895-9401-4f9c5a4c3dc8 Aparicio, Jesús F. f95ca308-8c47-4a0b-ac24-52dcf621928d 2026-02-04T12:26:27Z 2026-02-04T12:26:27Z 2004-05 0021-9193 https://hdl.handle.net/10259/11321 10.1128/JB.186.9.2567-2575.2004 1098-5530 Sequencing of the DNA region on the left fringe of the pimaricin gene cluster revealed the presence of a 3.6-kb gene, pimR, whose deduced product (1,198 amino acid residues) was found to have amino acid sequence homology with bacterial regulatory proteins. Database comparisons revealed that PimR represents the archetype of a new class of regulators, combining a Streptomyces antibiotic regulatory protein (SARP)-like N-terminal section with a C-terminal half homologous to guanylate cyclases and large ATP-binding regulators of the LuxR family. Gene replacement of pimR from Streptomyces natalensis chromosome results in a complete loss of pimaricin production, suggesting that PimR is a positive regulator of pimaricin biosynthesis. Gene expression analysis by reverse transcriptase PCR (RT-PCR) of the pimaricin gene cluster revealed that S. natalensis ΔPimR shows no expression at all of the cholesterol oxidase-encoding gene pimE, and very low level transcription of the remaining genes of the cluster except for the mutant pimR gene, thus demonstrating that this regulator activates the transcription of all the genes belonging to the pimaricin gene cluster but not its own transcription. This work was supported by a grant from the Comisio´n Interministerial de Ciencia y Tecnología (CICYT) to J.F.A. (BIO2001--0040). M.V.M. received a fellowship of the Fundac¸˜ao para a Cieˆncia e a Tecnologia (PRAXIS XXI/BD/15850/98). N.A. was the recipient of an F.P.U. fellowship from Ministerio de Educacio´n, Cultura, y Deporte (AP2002-1446). application/pdf eng American Society for Microbiology Journal of Bacteriology. 2004, V. 186, n. 9, p. 2567-2575 https://doi.org/10.1128/jb.186.9.2567-2575.2004 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Microbiología Bioquímica Biología molecular Genética Microbiology Biochemistry Molecular biology Genetics Identification of PimR as a Positive Regulator of Pimaricin Biosynthesis in Streptomyces natalensis info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Journal of Bacteriology 186 9 2567 2575 THUMBNAIL Anton-jb_2004.pdf.jpg Anton-jb_2004.pdf.jpg IM Thumbnail image/jpeg 4287 https://riubu.ubu.es/bitstream/10259/11321/4/Anton-jb_2004.pdf.jpg 32869167ebe34140788b85e81250ca8e MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/11321/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/11321/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Anton-jb_2004.pdf Anton-jb_2004.pdf application/pdf 865108 https://riubu.ubu.es/bitstream/10259/11321/1/Anton-jb_2004.pdf 65fb9cd0a84b4e555f22cc5284a42c35 MD5 1 10259/11321 oai:riubu.ubu.es:10259/11321 2026-02-05 02:05:55.403 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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