2026-05-08T08:15:09Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/115862026-05-07T00:05:37Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Santamaría Palacios, Jorge García, Nerea Rodríguez Lázaro, David 2026-05-06T11:29:26Z 2026-05-06T11:29:26Z 2025-01 1936-9751 https://hdl.handle.net/10259/11586 10.1007/s12161-024-02731-5 1936-976X The presence of the hepatitis E virus (HEV) in pork products, particularly in pig liver has been frequently described. However, a standardized method is not still available for the detection of HEV in foods, particularly in those difficult food matrixes such as pig meat and pork products. The aim of this study was to design, optimize and evaluate a new method of food-separation and virus concentration for HEV in pig liver samples. This method is based on organic flocculation and avoids the use of organic solvents. The virus recovery rates and analytical sensitivity of the method using murine norovirus MNV-1 as a surrogate were 73.6–82.2% and at least 1 × 103 TCID50 per g of liver in 100% of the replicates, respectively. Furthermore, this new methodology was validated by testing the presence of HEV RNA in naturally infected pig liver samples, comparing it with two other commonly used concentration methods. The new extraction method run satisfactory in comparison with the two reference methods; statistically equivalent (p < 0.05) to one of the methods used while presented statistically significant better results (p < 0.05) compared to the second method. Consequently, our results indicate that the new extraction method can be an adequate cost-effective and ecologically friendly alternative for the food separation and concentration of HEV RNA in pig liver samples. This study was supported by the research project PID2021-123532OB-C31 from the Spanish Ministry of Science and Innovation and the the research project BU220P24 from the Regional Government of Castilla y Leon. JSP received a pre-doctoral fellowship support from the Spanish Ministry of Science and Innovation FPU program (FPU2018/01313). Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. application/pdf eng Springer Food Analytical Methods. 2025, V. 18, n.5 https://doi.org/10.1007/s12161-024-02731-5 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Hepatitis E Virus Food Safety One Health Virus Extraction Pork Liver Organic Flocculation Virus detection RT-qPCR Virología veterinaria Veterinary virology An Efficient, Organic Solvent-Free Method for Extraction and Concentration of Hepatitis E Virus from Pig Liver info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Food Analytical Methods 18 5 767 775