2026-05-12T01:04:40Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/115982026-05-09T00:05:54Zcom_10259_7463com_10259_5086com_10259_2604col_10259_7464

Portugal Gómez, Paula 840 600 0000-0002-5669-5804 Svigelj, Rossella 63fccece-a61a-4e9c-b8ca-11bbfda48c64 600 Zanette, Fabiola 385806de-e217-47a1-90d1-98650e73ddad Toniolo, Rosanna efc83643-ab5d-4f03-a5b4-adcebbcca84a Domínguez Renedo, Olga 159 600 0000-0003-2067-0274 Alonso Lomillo, Mª Asunción 21 600 0000-0002-5714-3956 2026-05-08T10:41:02Z 2026-05-08T10:41:02Z 2025-05 0026-3672 https://hdl.handle.net/10259/11598 10.1007/s00604-025-07218-6 1436-5073 The use of environmentally friendly, cost-effective and biodegradable deep eutectic solvents (DESs) as nonaqueous solvents and electrolytes offers a promising avenue for enhancing enzymatic sensors and extending their applicability to the gas phase. In this study, an assembly is presented that includes a paper crown modified with tyrosinase enzyme, soaked in a DES and positioned on a disposable screen-printed carbon electrode. The paper crown contacts the outer edge of the carbon disk working electrode, as well as the peripheral counter and reference electrodes. This assembly yields a portable and disposable electrochemical platform, effortlessly immobilising DESs onto a porous and economical supporting material like paper. Moreover, the entire configuration resulted in a sensitive, rapidly responsive, membrane-free gas sensor whose response time depended exclusively on the enzymatic reaction. The electroanalytical capabilities of this setup were evaluated through voltammetric and amperometric determinations of phenols in synthetic and real wine samples. The proposed gas enzymatic sensor demonstrated excellent analytical performance for detecting phenolic compounds, offering fast measurement times, simple operation, high sensitivity, wide linear range and good repeatability. This research was supported by PID2023-149522OB-100 funded by MCIU/AEI/https://doi.org/10.13039/501100011033/FEDER,UE, and by the Italian Ministry of University and Research (PON 2014–2020, Action IV.6, 34-G-19900–3, project title “Smart Green Biosensors To Improve Food Sustainability and Environmental Preservation”). Open access funding provided by FEDER European Funds and the Junta de Castilla y León under the Research and Innovation Strategy for Smart Specialization (RIS3) of Castilla y León 2021-2027. application/pdf eng Springer Microchimica acta. 2025, V. 192, n. 6, 364 https://doi.org/10.1007/s00604-025-07218-6 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Phenols Screen-printed carbon electrodes Nanomaterials Gas sensor Deep eutectic solvents Wine analysis Vinos-análisis Wine and wine making-Analysis Gas sensor for 4-ethylguaiacol detection based on tyrosinase enzymatic activity in a deep eutectic solvent info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Microchimica Acta 192 6 THUMBNAIL Portugal-ma_2025.pdf.jpg Portugal-ma_2025.pdf.jpg IM Thumbnail image/jpeg 4632 https://riubu.ubu.es/bitstream/10259/11598/4/Portugal-ma_2025.pdf.jpg 4da1cc47f3a2d60ef6e7ff99015e1a90 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 1362 https://riubu.ubu.es/bitstream/10259/11598/3/license.txt 5d013bfa6e473ff0db22cd82a4d71a70 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/11598/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Portugal-ma_2025.pdf Portugal-ma_2025.pdf application/pdf 1490530 https://riubu.ubu.es/bitstream/10259/11598/1/Portugal-ma_2025.pdf 0c2beb569df3051f63c904bc015ce395 MD5 1 10259/11598 oai:riubu.ubu.es:10259/11598 2026-05-09 02:05:54.042 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es RWwgYXV0b3IgY29tbyDDum5pY28gdGl0dWxhciBkZSBsb3MgZGVyZWNob3MgZGUgcHJvcGllZGFkIGludGVsZWN0dWFsIGRlIGxhIG9icmEsIG8gZGlzcG9uaWVuZG8gZGUgbG9zIGRlYmlkb3MgcGVybWlzb3MgZGUgbG9zIG90cm9zIHRpdHVsYXJlcywgc2kgbG9zIGh1YmllcmEsIHkgZW4gdmlydHVkIGRlIGxvcyBkZXJlY2hvcyBxdWUgbGUgY29uZmllcmUgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3IsIApBVVRPUklaQSBhIGxhIFVuaXZlcnNpZGFkIGRlIEJ1cmdvcyBhIGRpZnVuZGlyLCBkZSBtYW5lcmEgZ3JhdHVpdGEsIGVsIGNvbnRlbmlkbyBkZSBsb3MgYXJjaGl2b3MgZGlnaXRhbGVzIHF1ZSBjb3JyZXNwb25kZW4gYWwgZG9jdW1lbnRvIGRlc2NyaXRvIGFudGVyaW9ybWVudGUsIGNvbiBjYXLDoWN0ZXIgbm8gZXhjbHVzaXZvIHkgZGUgbWFuZXJhIHDDumJsaWNhIGVuIGFjY2VzbyBhYmllcnRvIGEgdHJhdsOpcyBkZSBJbnRlcm5ldCwgcGFyYSBsbyBxdWUgbGEgQmlibGlvdGVjYSBwcm9jZWRlcsOhIGEgYXJjaGl2YXJsb3MgZW4gZWwgUmVwb3NpdG9yaW8gSW5zdGl0dWNpb25hbC4gQXNpbWlzbW8gYXV0b3JpemEgYSBsYSBVbml2ZXJzaWRhZCBkZSBCdXJnb3MgYSByZWFsaXphciBsYXMgdHJhbnNmb3JtYWNpb25lcyBuZWNlc2FyaWFzIGRlIGZvcm1hdG8sIG5vIGRlIGNvbnRlbmlkbywgcGFyYSBnYXJhbnRpemFyIGxhIHByZXNlcnZhY2nDs24geSBlbCBhY2Nlc28gZW4gZWwgZnV0dXJvLgoKRWwgYXV0b3IgZGlzcG9uZSwgZW4gdG9kbyBjYXNvLCBkZWwgZGVyZWNobyBhIHJldm9jYXIgZXN0YSBhdXRvcml6YWNpw7NuLgoKTGEgY2VzacOzbiBkZSBkZXJlY2hvcyBkZSBlc3RhIG9icmEgc2UgZW5jdWVudHJhIHN1amV0YSBhIGxhIGxlZ2lzbGFjacOzbiB2aWdlbnRlIHNvYnJlIHByb3BpZWRhZCBpbnRlbGVjdHVhbCB5IGRlcmVjaG9zIGRlIGF1dG9yLiBTdSBkaWZ1c2nDs24gZW4gZWwgUmVwb3NpdG9yaW8gc2Vyw6EgYmFqbyBsYSBtb2RhbGlkYWQgZGUgbGljZW5jaWEgQ3JlYXRpdmUgQ29tbW9ucyBvIGVxdWl2YWxlbnRlOiByZWNvbm9jaW1pZW50byDigJMgdXNvIG5vIGNvbWVyY2lhbCDigJMgc2luIG9icmEgZGVyaXZhZGEsIHBvciBsYSBxdWUgc2UgcGVybWl0ZSBoYWNlciBjb3BpYSwgZGlzdHJpYnVpciB5IGNvbXVuaWNhciBww7pibGljYW1lbnRlIGxhIG9icmEgc2llbXByZSBxdWUgc2UgY2l0ZSBhbCBhdXRvciwgZWwgdXNvIHF1ZSBzZSBoYWdhIGRlIGVsbGEgc2VhIG5vIGNvbWVyY2lhbCB5IG5vIHNlIGNyZWVuIG9icmFzIGRlcml2YWRhcyBhIHBhcnRpciBkZSBsYSBvcmlnaW5hbC4K