2026-04-28T12:22:51Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/43392022-12-19T11:00:07Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Melgosa Gómez, Rodrigo 357 500 0000-0003-2130-2422 Sanz Díez, Mª Teresa 530 500 0000-0003-1799-3099 García Solaesa, Ángela 51e8e08b-fee6-46e7-91e3-c3c9c3056196 500 Bucio López, Silvia Liliana 79 600 Beltrán Calvo, Sagrario 57 500 0000-0003-2559-3925 2017-02-13T11:17:22Z 2017-02-13T11:17:22Z 2015-02 0896-8446 http://hdl.handle.net/10259/4339 This work investigates the effect of supercritical carbon dioxide (SC-CO2) treatment on four commer-cial lipases. The influence of experimental conditions: temperature (35–70◦C), pressure (10–25 MPa),exposure time (1–3 h) and depressurization steps (1–3) on the residual activity was studied. Activityenhancement was verified for free enzymes (Palatase 20000 L and Lipozyme CALB L) treated at mildconditions; while the highest temperature and pressure and the longest exposure time assayed led toactivity losses. On the other hand, activity losses or no significative effect of the treatment in the enzymeactivity were observed on immobilized enzymes (Lipozyme RM IM and Lipozyme 435). Qualitative stud-ies were also performed: Fluorescence emission spectra showed changes in the conformational structureof both two free enzymes after SC-CO2treatment. Scanning electron micrographs showed morphologicalalterations in the immobilization supports of the treated enzymes; while infrared spectra did not showchemical modifications. (MINECO) and the EuropeanRegional Development Fund (ERDF) for financial support to theproject CTQ2012-39131-C02-01. application/pdf eng Elsevier The Journal of Supercritical Fluids. 2015, V. 97, p. 51–62 http://dx.doi.org/10.1016/j.supflu.2014.11.003 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Candida antarctica lipase B Rhizomucor miehei lipase Immobilized lipase Lipozyme Enzyme activity Supercritical carbon dioxide Chemical engineering Ingeniería química Enzymatic activity and conformational and morphological studies of four commercial lipases treated with supercritical carbon dioxide info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/4339/8/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 8 THUMBNAIL Melgosa-JSF_2015.pdf.jpg Melgosa-JSF_2015.pdf.jpg IM Thumbnail image/jpeg 3819 https://riubu.ubu.es/bitstream/10259/4339/7/Melgosa-JSF_2015.pdf.jpg 94a463f7cf8bd6385e6e52a10e240d5d MD5 7 ORIGINAL Melgosa-JSF_2015.pdf Melgosa-JSF_2015.pdf application/pdf 1225126 https://riubu.ubu.es/bitstream/10259/4339/1/Melgosa-JSF_2015.pdf 87a63ac2aa5ee820c88d550176bea144 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/4339/5/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 5 TEXT Melgosa-JSF_2015.pdf.txt Melgosa-JSF_2015.pdf.txt Extracted text text/plain 43672 https://riubu.ubu.es/bitstream/10259/4339/6/Melgosa-JSF_2015.pdf.txt c779d12536ed085865414141d5d53888 MD5 6 10259/4339 oai:riubu.ubu.es:10259/4339 2022-12-19 12:00:07.025 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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