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oai:riubu.ubu.es:10259/43612022-12-19T13:18:42Zcom_10259_4354com_10259_5086com_10259_2604com_10259_4363com_10259_4365col_10259_4355col_10259_4364col_10259_4366

Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes Díaz de Greñu Puertas, Borja García Calvo, José Cuevas Vicario, José Vicente García Herbosa, Gabriel García Ruiz, Begoña Busto Vázquez, Natalia Ibeas Cortes, Saturnino Torroba Pérez, Tomás Torroba, Blanca Herrera, Antonio Pons, Sebastian Chemistry, Organic Química orgánica Selected new fluorogenic probes that interact in different ways with Hg2+ and MeHg+ have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg2+ and MeHg+ has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg2+, MeHg+ and the fluorogenic probes. In particular, MeHg+ was selectively detected in cell nuclei by probe JG45. the Ministerio de Econom´ıa y Competitividad, Spain (Projects CTQ2012- 31611 and CTQ2014-58812-C2-2-R), Junta de Castilla y Le´on, Consejer´ıa de Educaci´on y Cultura y Fondo Social Europeo (Project BU246A12-1), Obra Social “la Caixa” OSLC-2012-007 and the European Commission, Seventh Framework Programme (Project SNIFFER FP7-SEC-2012-312411). 2017-03-14T11:36:48Z 2017-03-14T11:36:48Z 2015-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/10259/4361 eng Chemical Science. 2015, V. 6, n. 7, p. 3757-3764 http://dx.doi.org/10.1039/C5SC00718F info:eu-repo/grantAgreement/EC/FP7/312411 Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess application/pdf Royal Society of Chemistry