2026-05-05T07:47:52Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/43812022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377

Alvarado Gámez, Ana Lorena d83ef3b7-a53a-4ad5-99b6-f1d3b01baeaa 500 Alonso Lomillo, Mª Asunción 21 500 Domínguez Renedo, Olga 159 500 Arcos Martínez, Julia 36 500 0000-0002-4932-6445 2017-03-20T09:29:11Z 2017-03-20T09:29:11Z 2014-02 http://hdl.handle.net/10259/4381 A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water Research Vicerrectory of Costa Rica University (Project 804-B0-058) and Spanish Ministry of Science and Innovation (TEC-2009/12029). This work was supported by the Spanish Ministry of Science and Innovation (MICINN) 410 and the European Regional Development Fund (FEDER) (INNPACTO SERIBIO 2011-411 2014) and TEC2009-12029, as well as through Junta de Castilla y León (BU212A12-2). application/pdf eng MDPI Sensors, 2014, V. 14, n. 2, p. 3756-3767 http://dx.doi.org/10.3390/s140203756 Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess alkaline phosphatase biosensor gold nanoparticles 4-nitrophenyl phosphate screen printed electrode vanadium water analysis Chemistry, Analytic Química analítica A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/4381/8/license_rdf f9a6a4096bf314846151c97900b2090d MD5 8 THUMBNAIL Alvarado-Sensors_2014.pdf.jpg Alvarado-Sensors_2014.pdf.jpg IM Thumbnail image/jpeg 4546 https://riubu.ubu.es/bitstream/10259/4381/7/Alvarado-Sensors_2014.pdf.jpg c0c370e5d235c03955e009f454ea43ac MD5 7 ORIGINAL Alvarado-Sensors_2014.pdf Alvarado-Sensors_2014.pdf application/pdf 376513 https://riubu.ubu.es/bitstream/10259/4381/1/Alvarado-Sensors_2014.pdf bacecc1fdc15bfb3c5f337cfade4f5b0 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/4381/5/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 5 TEXT Alvarado-Sensors_2014.pdf.txt Alvarado-Sensors_2014.pdf.txt Extracted text text/plain 31287 https://riubu.ubu.es/bitstream/10259/4381/6/Alvarado-Sensors_2014.pdf.txt e55bdacb8de8ecd8a610a7a7c97df9fe MD5 6 10259/4381 oai:riubu.ubu.es:10259/4381 2022-04-29 14:02:47.097 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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