2026-04-28T11:35:25Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/44062022-04-29T12:02:47Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863
Quijada, Narciso M.
Fongaro, Gislaine
Barardi, Célia R. M.
Hernández Pérez, Marta
Rodríguez Lázaro, David
2017-04-06T07:23:11Z
2017-04-06T07:23:11Z
2016-12
http://hdl.handle.net/10259/4406
The increase of foodborne viral outbreaks highlights the need for a rapid and sensitive
method for the prediction of viral infectivity in food samples. This study assesses the use
of propidium monoazide (PMA) coupled with real-time PCR methods (RT-qPCR or qPCR
for RNA or DNA viruses, respectively) in the determination of viral infectivity in complex
animal-related food matrices. Clam and Spanish fermented sausage (“chorizo”) samples
were spiked with infectious and heat-inactivated human adenovirus-2 (HAdV-2) and
mengovirus (vMC0). PMA-qPCR/RT-qPCR discriminated infective virus particles, with
significant reductions (>2.7 log10 or 99.7%). Additionally, infectious HAdV-2 and vMC0
were quantified by plaque assay (in plaque forming units, PFU), and compared with
those in virus genomes copies (GCs) quantified by PMA-qPCR/RT-qPCR. A consistent
correlation (R2 > 0.92) was showed between PFU and GCs along serial 10-fold dilutions
in both DNA and RNA virus and in both food matrices. This study shows the use of PMA
coupled to qPCR/RT-qPCR as a promising alternative for prediction of viral infectivity in
food samples in comparison to more expensive and time-consuming methods and for
those viruses that are not able to grow under available cell culture techniques.
ThisstudywasfinanciallysupportedbytheRTA2014-00024-
C04-01 fromtheSpanishMinistryofEconomyandInnovation
and theBrazilianCNPqProjectnumber472804/2013-8,andby
CAPES/PNPD andCAPES/PDSE.
application/pdf
eng
Frontiers Media
Frontiers in Microbiology. 2016, V. 7, art. 2008
https://doi.org/10.3389/fmicb.2016.02008
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
enteric viruses
infectivity prediction
PMA-PCR
clam
fermented sausage
Microbiology
Microbiología
Propidium Monoazide Integrated with qPCR Enables the Detection and Enumeration of Infectious Enteric RNA and DNA Viruses in Clam and Fermented Sausages
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion