2026-04-28T15:39:55Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/44062022-04-29T12:02:47Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863
Repositorio Institucional de la Universidad de Burgos
author
Quijada, Narciso M.
author
Fongaro, Gislaine
author
Barardi, Célia R. M.
author
Hernández Pérez, Marta
author
Rodríguez Lázaro, David
2017-04-06T07:23:11Z
2017-04-06T07:23:11Z
2016-12
http://hdl.handle.net/10259/4406
The increase of foodborne viral outbreaks highlights the need for a rapid and sensitive
method for the prediction of viral infectivity in food samples. This study assesses the use
of propidium monoazide (PMA) coupled with real-time PCR methods (RT-qPCR or qPCR
for RNA or DNA viruses, respectively) in the determination of viral infectivity in complex
animal-related food matrices. Clam and Spanish fermented sausage (“chorizo”) samples
were spiked with infectious and heat-inactivated human adenovirus-2 (HAdV-2) and
mengovirus (vMC0). PMA-qPCR/RT-qPCR discriminated infective virus particles, with
significant reductions (>2.7 log10 or 99.7%). Additionally, infectious HAdV-2 and vMC0
were quantified by plaque assay (in plaque forming units, PFU), and compared with
those in virus genomes copies (GCs) quantified by PMA-qPCR/RT-qPCR. A consistent
correlation (R2 > 0.92) was showed between PFU and GCs along serial 10-fold dilutions
in both DNA and RNA virus and in both food matrices. This study shows the use of PMA
coupled to qPCR/RT-qPCR as a promising alternative for prediction of viral infectivity in
food samples in comparison to more expensive and time-consuming methods and for
those viruses that are not able to grow under available cell culture techniques.
eng
Attribution 4.0 International
enteric viruses
infectivity prediction
PMA-PCR
clam
fermented sausage
Propidium Monoazide Integrated with qPCR Enables the Detection and Enumeration of Infectious Enteric RNA and DNA Viruses in Clam and Fermented Sausages
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/4406/1/Quijada-FM_2016.pdf
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https://riubu.ubu.es/bitstream/10259/4406/6/Quijada-FM_2016.pdf.txt
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Quijada-FM_2016.pdf.txt