2026-05-12T06:44:40Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/44072022-04-29T12:02:47Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Different Behavior of Enteric Bacteria and Viruses in Clay and Sandy Soils after Biofertilization with Swine Digestate Fongaro, Gislaine García González, María C. Hernández Pérez, Marta Kunz, Airton Barardi, Célia R. M. Rodríguez Lázaro, David swine digestate clay and sandy soils biofertilization biomarkers management Microbiology Microbiología Enteric pathogens from biofertilizer can accumulate in the soil, subsequently contaminating water and crops. We evaluated the survival, percolation and leaching of model enteric pathogens in clay and sandy soils after biofertilization with swine digestate: PhiX-174, mengovirus (vMC0), Salmonella enterica Typhimurium and Escherichia coli O157:H7 were used as biomarkers. The survival of vMC0 and PhiX-174 in clay soil was significantly lower than in sandy soil (ıPT90 values of 10.520 0.600 vs. 21.270 1.100 and 12.040 0.010 vs. 43.470 1.300, respectively) and PhiX- 174 showed faster percolation and leaching in sandy soil than clay soil (ıPT90 values of 0.46 and 2.43, respectively). S. enterica Typhimurium was percolated and inactivated more slowly than E. coli O157:H7 (ıPT90 values of 9.340 0.200 vs. 6.620 0.500 and 11.900 0.900 vs. 10.750 0.900 in clay and sandy soils, respectively), such that E. coli O157:H7 was transferred more quickly to the deeper layers of both soils evaluated (percolation). Our findings suggest that E. coli O157:H7 may serve as a useful microbial biomarker of depth contamination and leaching in clay and sandy soil and that bacteriophage could be used as an indicator of enteric pathogen persistence. Our study contributes to development of predictive models for enteric pathogen behavior in soils, and for potential water and food contamination associated with biofertilization, useful for risk management and mitigation in swine digestate recycling. RTA2014-00024-C04-01 from the Spanish Ministry of Economy and Innovation and the Brazilian CNPq Project number 472804/2013-8, an dby CAPES/PNPD and CAPES/PDSE. 2017-04-06T07:48:07Z 2017-04-06T07:48:07Z 2017-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/10259/4407 eng Frontiers in Microbiology. 2017, V. 8, art. 74 https://doi.org/10.3389/fmicb.2017.00074 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess application/pdf Frontiers Media