2026-04-28T19:13:37Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/45672022-04-29T12:02:46Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Melgosa Gómez, Rodrigo 357 500 Sanz Díez, Mª Teresa 530 500 García Solaesa, Ángela 219 500 Paz Barragán, Esther de 421 500 Beltrán Calvo, Sagrario 57 500 Lamas, Daniela L. b8dae0ee-86ba-4d27-84ab-75d8c7400078 500 2017-08-24T10:15:51Z 2019-01-01T03:45:06Z 2017-01 2212-9820 http://hdl.handle.net/10259/4567 10.1016/j.jcou.2016.11.011 Supercritical carbon dioxide (SC-CO2) has been used as green solvent in the lipase-catalyzed ethanolysis of fish oil by Lipozyme RM IM at mild, non-oxidative conditions and with no solvent residues. The effect of experimental conditions, initial substrate ethanol/oil molar ratio (2–38), pressure (7.5–30 MPa), and temperature (323.15–353.15 K) on equilibrium conversion, reaction rate and oxidative status of the products has been studied. No ethanol inhibition has been observed at high concentrations of ethanol, when putting in contact first the fish oil with the enzyme avoiding direct contact between the biocatalyst and ethanol. Operating pressure affected positively the reaction performance in the range investigated. Visual observation of the phase behaviour of the initial reaction mixture showed an “expanded liquid phase” that helped enhancing reaction rate, and a gas phase. Raising temperature accelerated the reaction up to a limit (343.15 K), observing higher enzyme thermal stability than in other reaction media (313.15 K). However, lipid oxidation increases with temperature. Up to 86 ± 1% FAEE yield has been found at MR = 6:1, 30 MPa and 323.15 K. Kinetic data have been correlated by using a mathematical model based on the elementary reactions of the 3-step transesterification. Kinetic rate constants, apparent activation volumes and energies are reported for the first time for a lipase-catalyzed ethanolysis reaction in SC-CO2. Spanish Government (MINECO) and the European Regional Development Fund (ERDF) for financial support of the project CTQ2012-39131-C02-01. Financial support from the Junta de Castilla y León and European Regional Development Fund (ERDF) through project BU055U16 application/pdf eng Elsevier Journal of CO2 Utilization. 2017, V. 17, p. 170–179 https://doi.org/10.1016/j.jcou.2016.11.011 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Omega 3 Lipase Ethanolysis Supercritical carbon dioxide Chemical engineering Ingeniería química Supercritical carbon dioxide as solvent in the lipase-catalyzed ethanolysis of fish oil: kinetic study info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/4567/8/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 8 THUMBNAIL Melgosa-JCO2U_2017.pdf.jpg Melgosa-JCO2U_2017.pdf.jpg IM Thumbnail image/jpeg 3925 https://riubu.ubu.es/bitstream/10259/4567/7/Melgosa-JCO2U_2017.pdf.jpg 0967121526f9c374a5485f927dacbfb5 MD5 7 ORIGINAL Melgosa-JCO2U_2017.pdf Melgosa-JCO2U_2017.pdf application/pdf 1984560 https://riubu.ubu.es/bitstream/10259/4567/1/Melgosa-JCO2U_2017.pdf 093136f9fd89a63d7fd836dbcef90588 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/4567/5/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 5 TEXT Melgosa-JCO2U_2017.pdf.txt Melgosa-JCO2U_2017.pdf.txt Extracted text text/plain 54469 https://riubu.ubu.es/bitstream/10259/4567/6/Melgosa-JCO2U_2017.pdf.txt b62b312e4642892241e56594e5af81c5 MD5 6 10259/4567 oai:riubu.ubu.es:10259/4567 2022-04-29 14:02:46.909 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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