2026-05-07T07:25:01Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/47242023-10-02T08:19:52Zcom_10259.4_2551com_10259_5086com_10259_2604col_10259.4_2552
Wadsworth, Caroline .
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500
Procopio, Noemi .
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500
Anderung, Cecilia .
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500
Carretero Díaz, José Miguel
106
600
0000-0003-0409-8087
Iriarte Avilés, Eneko
297
600
0000-0001-8365-5616
Valdiosera Morales, Cristina Eugenia
716
600
0000-0003-4948-2226
Rengert Elburg .
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500
Kirsty Penkman .
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Buckley, Michael
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2018-02-02T12:20:48Z
2018-03-01T03:45:06Z
2017-03
1874-3919
http://hdl.handle.net/10259/4724
10.1016/j.jprot.2017.01.004
Ancient DNA (aDNA) is themost informative biomolecule extracted fromskeletal remains at archaeological sites,
but its survival is unpredictable and its extraction and analysis is time consuming, expensive and often fails. Several
proposed methods for better understanding aDNA survival are based upon the characterisation of some aspect
of protein survival, but these are typically non-specific; proteomic analyses may offer an attractive method
for understanding preservation processes. In this study, in-depth proteomic (LC-Orbitrap-MS/MS) analyseswere
carried out on 69 archaeological bovine bone and dentine samples from multiple European archaeological sites
and comparedwith mitochondrial aDNA and amino acid racemisation (AAR) data. Comparisons of these data, including
estimations of the relative abundances for seven selected non-collagenous proteins, indicate that the survival
of aDNA in bone or dentine may correlatewith the survival of some proteins, and that proteome complexity
is a more useful predictor of aDNA survival than protein abundance or AAR. The lack of a strong correlation between
the recovery of aDNA and the proteome abundance may indicate that the survival of aDNA is more closely
linked to its ability to associate with bone hydroxyapatite crystals rather than to associate with proteins.
Significance: Ancient biomolecule survival remains poorly understood, even with great advancements in ‘omics’
technologies, both in genomics and proteomics. This study investigates the survival of ancient DNA in relation to
that of proteins, taking into account proteome complexity and the relative protein abundances to improve our
understanding of survival mechanisms. The results show that although protein abundance is not necessarily directly
related to aDNA survival, proteome complexity appears to be.
NERC for funding the studentship to CW (NE/J500057/1) and the Royal Society for funding for MB (UF120473 and UF120473). Assistant Professor (CA) funded by the Swedish Research Council Formas (2010-627) and KP by the Leverhulme Trust (PLP-2012-116). The Atapuerca research was supported by the Spanish MINECO (CGL2012-38434-C03-01) project. Fieldwork at the Atapuerca sites was funded by the Junta de Castilla y León and the Fundación Atapuerca.
application/pdf
eng
Elsevier
Journal of Proteomics.2017, V. 158, p. 1-8
https://doi.org/10.1016/j.jprot.2017.01.004
info:eu-repo/grantAgreement/MINECO/CGL2012-38434-C03-01
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Ancient DNA
Ancient proteins
Proteomics
Collagen
Non-collagenous proteins
Paleontology
Paleontología
Comparing ancient DNA survival and proteome content in 69 archaeological cattle tooth and bone samples from multiple European sites
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
CC-LICENSE
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oai:riubu.ubu.es:10259/4724
2023-10-02 10:19:52.301
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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