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oai:riubu.ubu.es:10259/47462022-04-29T12:02:48Zcom_10259_4365com_10259_5086com_10259_2604com_10259_4883col_10259_4366col_10259_4884

Pérez Arnáiz, Cristina Busto Vázquez, Natalia Santolaya, Javier Leal Villalba, José María Barone, Giampaolo García Ruiz, Begoña Tel22 conformations TMPyP4 Fast reactions Molecular dynamics Background: Stabilization of G-quadruplex helices by small ligands has attracted growing attention because they inhibit the activity of the enzyme telomerase, which is overexpressed in> 80% cancer cells. TMPyP4, one of the most studied G-quadruplex ligands, is used as a model to show that the ligands can exhibit different binding features with different conformations of a human telomeric specific sequence. Methods: UV–Vis, FRET melting Assay, Isothermal Titration Calorimetry, Time-resolved Fluorescence lifetime, T-Jump and Molecular Dynamics. Results: TMPyP4 yields two different complexes with two Tel22 telomeric conformations in the presence of Na+ or K+. T-Jump kinetic experiments show that the rates of formation and dissociation of these complexes in the ms time scale differ by one order of magnitude. MD simulations reveal that, in K+ buffer, “hybrid 1” conformation yields kinetic constants on interaction with TMPyP4 one order lower than “hybrid 2”. The binding involves π–π stacking with external loop bases. Conclusions: For the first time we show that for a particular buffer TMPyP4 interacts in a kinetically different way with the two Tel22 conformations even if the complexes formed are thermodynamically indistinguishable. General significance: G-quadruplexes, endowed with technological applications and potential impact on regulation mechanisms, define a new research field. The possibility of building different conformations from same sequence is a complex issue that confers G-quadruplexes very interesting features. The obtaining of reliable kinetic data constitutes an efficient tool to determine reaction mechanisms between conformations and small molecules. 2018-03-08 2019-03-01 2018-03 info:eu-repo/semantics/article 0304-4165 http://hdl.handle.net/10259/4746 10.1016/j.bbagen.2017.10.020 eng Biochimica et biophysica acta (BBA) - general subjects. 2018, V. 1862, n. 3, p. 522-531 https://doi.org/10.1016/j.bbagen.2017.10.020 info:eu-repo/grantAgreement/“la Caixa” Foundation/OSLC-2012-007 info:eu-repo/grantAgreement/MINECO/CTQ2014-58812-C2-2-R info:eu-repo/grantAgreement/JCyL/BU042U16 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier