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oai:riubu.ubu.es:10259/48822021-11-10T09:38:24Zcom_10259_4365com_10259_5086com_10259_2604com_10259_4354com_10259_4883col_10259_4366col_10259_4355col_10259_4884

Busto Vázquez, Natalia Calvo, Patricia Santolaya, Javier Leal Villalba, José María Guédin, Aurore . Barone, Giampaolo Torroba Pérez, Tomás Mergny, Jean-Louis García Ruiz, Begoña 2018-08-24T11:28:04Z 2019-08-06T02:45:07Z 2018-08 0947-6539 http://hdl.handle.net/10259/4882 10.1002/chem.201802365 A new fluorescent, non‐cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G‐quadruplexes with scarce or no affinity towards single‐ or double‐stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G‐quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G‐quadruplex structures from solution, even in the presence of an excess of single‐stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2‐ over 3‐tetrad quadruplexes, which constitutes a novel type of behavior. “la Caixa” Foundation (LCF/PR/PR12/ 11070003), MINECO (CTQ2014-58812-C2-2-R and CTQ2015- 71353-R, FEDER Funds), and Junta de Castilla y Lejn, Consejer &a de Educacijn y Cultura y Fondo Social Europeo (Project BU051U16), Spain, is gratefully acknowledged. N.B. is grateful to Dr. Oscar Mendoza of the ARNA Laboratory for useful discussion and also to the financial support of the Jos8 Castillejo Program by the Spanish Ministry of Education, Culture and Sports (JC2015-00403). J.L.M. acknowledges support from Conseil Regional d’Aquitaine, Agence Nationale de la Recherche (ANR Quarpdiem) and the Symbit program [CZ.02.1.01/0.0/0.0/ 15_003/0000477] financed by the ERDF. application/pdf eng Wiley-VCH Verlag Chemistry-a european journal. 2018, V. 24, n. 44, p. 11292-11296 https://doi.org/10.1002/chem.201802365 info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF-PR-PR12-11070003 info:eu-repo/grantAgreement/MINECO/CTQ2014-58812-C2-2-R info:eu-repo/grantAgreement/MINECO/CTQ2015-71353-R info:eu-repo/grantAgreement/JCyL/BU051U16 Química Chemistry Fishing for G-Quadruplexes in solution with a perylene diimide derivative labeled with biotins info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess