2026-04-17T03:05:52Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/48822021-11-10T09:38:24Zcom_10259_4365com_10259_5086com_10259_2604com_10259_4354com_10259_4883col_10259_4366col_10259_4355col_10259_4884
Repositorio Institucional de la Universidad de Burgos
author
Busto Vázquez, Natalia
author
Calvo, Patricia
author
Santolaya, Javier
author
Leal Villalba, José María
author
Guédin, Aurore .
author
Barone, Giampaolo
author
Torroba Pérez, Tomás
author
Mergny, Jean-Louis
author
García Ruiz, Begoña
2018-08-24T11:28:04Z
2019-08-06T02:45:07Z
2018-08
0947-6539
http://hdl.handle.net/10259/4882
10.1002/chem.201802365
A new fluorescent, non‐cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G‐quadruplexes with scarce or no affinity towards single‐ or double‐stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G‐quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G‐quadruplex structures from solution, even in the presence of an excess of single‐stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2‐ over 3‐tetrad quadruplexes, which constitutes a novel type of behavior.
eng
Fishing for G-Quadruplexes in solution with a perylene diimide derivative labeled with biotins
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/4882/1/Busto-Chemistry_2018.pdf
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https://riubu.ubu.es/bitstream/10259/4882/3/Busto-Chemistry_2018.pdf.txt
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Busto-Chemistry_2018.pdf.txt