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oai:riubu.ubu.es:10259/48822021-11-10T09:38:24Zcom_10259_4365com_10259_5086com_10259_2604com_10259_4354com_10259_4883col_10259_4366col_10259_4355col_10259_4884

Fishing for G-Quadruplexes in solution with a perylene diimide derivative labeled with biotins Busto Vázquez, Natalia Calvo, Patricia Santolaya, Javier Leal Villalba, José María Guédin, Aurore . Barone, Giampaolo Torroba Pérez, Tomás Mergny, Jean-Louis García Ruiz, Begoña A new fluorescent, non‐cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G‐quadruplexes with scarce or no affinity towards single‐ or double‐stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G‐quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G‐quadruplex structures from solution, even in the presence of an excess of single‐stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2‐ over 3‐tetrad quadruplexes, which constitutes a novel type of behavior. 2019-08-06T02:45:07Z 2019-08-06T02:45:07Z 2019-08-06T02:45:07Z 2018-08 info:eu-repo/semantics/article 0947-6539 http://hdl.handle.net/10259/4882 10.1002/chem.201802365 eng Chemistry-a european journal. 2018, V. 24, n. 44, p. 11292-11296 https://doi.org/10.1002/chem.201802365 info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF-PR-PR12-11070003 info:eu-repo/grantAgreement/MINECO/CTQ2014-58812-C2-2-R info:eu-repo/grantAgreement/MINECO/CTQ2015-71353-R info:eu-repo/grantAgreement/JCyL/BU051U16 info:eu-repo/semantics/openAccess Wiley-VCH Verlag