2026-04-17T04:23:46Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/48822021-11-10T09:38:24Zcom_10259_4365com_10259_5086com_10259_2604com_10259_4354com_10259_4883col_10259_4366col_10259_4355col_10259_4884

Busto Vázquez, Natalia 83 500 Calvo, Patricia 94 500 Santolaya, Javier ac73c679-73ee-420a-b52e-e67a1b6502d7 500 Leal Villalba, José María 314 500 Guédin, Aurore . 65cdf1a5-a7df-4e80-99e0-97dce80a9f83 500 Barone, Giampaolo 2502c306-a514-4e96-9cd8-7a3b547ae347 500 Torroba Pérez, Tomás 554 500 Mergny, Jean-Louis 3e0fe3f8-e25a-4a52-bd7b-bc8000a6e158 500 García Ruiz, Begoña 217 500 2018-08-24T11:28:04Z 2019-08-06T02:45:07Z 2018-08 0947-6539 http://hdl.handle.net/10259/4882 10.1002/chem.201802365 A new fluorescent, non‐cytotoxic perylene diimide derivative with two biotins at the peri position, PDI2B, has been synthesized. This molecule is able to interact selectively with G‐quadruplexes with scarce or no affinity towards single‐ or double‐stranded DNA. These features have made it possible to design a simple, effective, safe, cheap, and selective method for fishing G‐quadruplex structures in solution by use of PDI2B and streptavidin coated magnetic beads. The new cyclic method reported leads to the recovery of more than 80 % of G‐quadruplex structures from solution, even in the presence of an excess of single‐stranded or duplex DNA as competitors. Moreover, PDI2B is a G4 ligand that can display higher thermal stabilization and greater affinity for 2‐ over 3‐tetrad quadruplexes, which constitutes a novel type of behavior. “la Caixa” Foundation (LCF/PR/PR12/ 11070003), MINECO (CTQ2014-58812-C2-2-R and CTQ2015- 71353-R, FEDER Funds), and Junta de Castilla y Lejn, Consejer &a de Educacijn y Cultura y Fondo Social Europeo (Project BU051U16), Spain, is gratefully acknowledged. N.B. is grateful to Dr. Oscar Mendoza of the ARNA Laboratory for useful discussion and also to the financial support of the Jos8 Castillejo Program by the Spanish Ministry of Education, Culture and Sports (JC2015-00403). J.L.M. acknowledges support from Conseil Regional d’Aquitaine, Agence Nationale de la Recherche (ANR Quarpdiem) and the Symbit program [CZ.02.1.01/0.0/0.0/ 15_003/0000477] financed by the ERDF. application/pdf eng Wiley-VCH Verlag Chemistry-a european journal. 2018, V. 24, n. 44, p. 11292-11296 https://doi.org/10.1002/chem.201802365 info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF-PR-PR12-11070003 info:eu-repo/grantAgreement/MINECO/CTQ2014-58812-C2-2-R info:eu-repo/grantAgreement/MINECO/CTQ2015-71353-R info:eu-repo/grantAgreement/JCyL/BU051U16 Química Chemistry Fishing for G-Quadruplexes in solution with a perylene diimide derivative labeled with biotins info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess THUMBNAIL Busto-Chemistry_2018.pdf.jpg Busto-Chemistry_2018.pdf.jpg IM Thumbnail image/jpeg 3688 https://riubu.ubu.es/bitstream/10259/4882/4/Busto-Chemistry_2018.pdf.jpg a63c240fd95de726090dc7d0d1fe67c0 MD5 4 ORIGINAL Busto-Chemistry_2018.pdf Busto-Chemistry_2018.pdf application/pdf 2004040 https://riubu.ubu.es/bitstream/10259/4882/1/Busto-Chemistry_2018.pdf a4ca0185658afe4975e50d7ae0d3ed63 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/4882/2/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 2 TEXT Busto-Chemistry_2018.pdf.txt Busto-Chemistry_2018.pdf.txt Extracted text text/plain 24062 https://riubu.ubu.es/bitstream/10259/4882/3/Busto-Chemistry_2018.pdf.txt ee5ca42dfc136626f1c5ae9eb53b22cb MD5 3 10259/4882 oai:riubu.ubu.es:10259/4882 2021-11-10 10:38:24.284 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es RWwgYXV0b3IgY29tbyDDum5pY28gdGl0dWxhciBkZSBsb3MgZGVyZWNob3MgZGUgcHJvcGllZGFkIGludGVsZWN0dWFsIGRlIGxhIG9icmEsIG8gZGlzcG9uaWVuZG8gZGUgbG9zIGRlYmlkb3MgcGVybWlzb3MgZGUgbG9zIG90cm9zIHRpdHVsYXJlcywgc2kgbG9zIGh1YmllcmEsIHkgZW4gdmlydHVkIGRlIGxvcyBkZXJlY2hvcyBxdWUgbGUgY29uZmllcmUgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3IsIA0KQVVUT1JJWkEgYSBsYSBVbml2ZXJzaWRhZCBkZSBCdXJnb3MgYSBkaWZ1bmRpciwgZGUgbWFuZXJhIGdyYXR1aXRhLCBlbCBjb250ZW5pZG8gZGUgbG9zIGFyY2hpdm9zIGRpZ2l0YWxlcyBxdWUgY29ycmVzcG9uZGVuIGFsIGRvY3VtZW50byBkZXNjcml0byBhbnRlcmlvcm1lbnRlLCBjb24gY2Fyw6FjdGVyIG5vIGV4Y2x1c2l2byB5IGRlIG1hbmVyYSBww7pibGljYSBlbiBhY2Nlc28gYWJpZXJ0byBhIHRyYXbDqXMgZGUgSW50ZXJuZXQsIHBhcmEgbG8gcXVlIGxhIEJpYmxpb3RlY2EgcHJvY2VkZXLDoSBhIGFyY2hpdmFybG9zIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwuIEFzaW1pc21vIGF1dG9yaXphIGEgbGEgVW5pdmVyc2lkYWQgZGUgQnVyZ29zIGEgcmVhbGl6YXIgbGFzIHRyYW5zZm9ybWFjaW9uZXMgbmVjZXNhcmlhcyBkZSBmb3JtYXRvLCBubyBkZSBjb250ZW5pZG8sIHBhcmEgZ2FyYW50aXphciBsYSBwcmVzZXJ2YWNpw7NuIHkgZWwgYWNjZXNvIGVuIGVsIGZ1dHVyby4NCg0KRWwgYXV0b3IgZGlzcG9uZSwgZW4gdG9kbyBjYXNvLCBkZWwgZGVyZWNobyBhIHJldm9jYXIgZXN0YSBhdXRvcml6YWNpw7NuLg0KDQpMYSBjZXNpw7NuIGRlIGRlcmVjaG9zIGRlIGVzdGEgb2JyYSBzZSBlbmN1ZW50cmEgc3VqZXRhIGEgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3Iu