2026-05-05T01:19:25Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/50452021-11-10T09:38:20Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Benito Román, Oscar 59 500 0000-0002-4418-0045 Sanz Díez, Mª Teresa 530 500 Illera Gigante, Alba Ester 296 500 Melgosa Gómez, Rodrigo 357 500 Benito Moreno, José Manuel 58 500 Beltrán Calvo, Sagrario 57 500 2019-01-08T12:10:08Z 2019-03 2021-04-01 0896-8446 http://hdl.handle.net/10259/5045 10.1016/j.supflu.2018.11.009 In the present work the effect of High Pressure Carbon Dioxide (HPCD) on the activity and structure of a commercial pectin methylesterase (PME) was studied and the results were compared to the conventional thermal inactivation technology. The use of supercritical CO2 (pressure 6–18 MPa, temperature 40–55 °C and time up to 75 min) increased dramatically the PME inactivation rate. The experimental data fitted the first order model and the inactivation kinetic study of PME was completed with the calculations of the activation energy and volume of activation. HPCD treatment induced important changes in the tertiary structure of the enzyme, as revealed the intrinsic fluorescence, KI quenching and ANS binding analyses. They showed that HPCD treatment rearranged the structure of the enzyme. These changes induced by HPCD were significantly different from those induced by mild thermal treatment. Finally, the structural changes detected correlated with the enzyme activity losses observed. Spanish Ministry of Economy and Competitiveness for financial support of project CTQ2015-64396-R and A.E. Illera’s contract. To Junta de Castilla y León and ERDF for financial support of project BU055U16 and O. Benito-Román’s Post-doctoral contract. To Spanish Mi application/pdf eng Elsevier The Journal of Supercritical Fluids. 2019, V. 145, p. 111-121 https://doi.org/10.1016/j.supflu.2018.11.009 info:eu-repo/grantAgreement/MINECO/CTQ2015-64396-R info:eu-repo/grantAgreement/JCyL/BU055U16 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess PME HPCD Enzyme inactivation CO2 Juice Ingeniería química Chemical engineering Pectin methylesterase inactivation by High Pressure Carbon Dioxide (HPCD) info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/5045/8/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 8 THUMBNAIL Benito-tjsf_2019.pdf.jpg Benito-tjsf_2019.pdf.jpg IM Thumbnail image/jpeg 3643 https://riubu.ubu.es/bitstream/10259/5045/7/Benito-tjsf_2019.pdf.jpg 3f311dfaecc79f618f6a8b529150561c MD5 7 ORIGINAL Benito-tjsf_2019.pdf Benito-tjsf_2019.pdf application/pdf 743159 https://riubu.ubu.es/bitstream/10259/5045/1/Benito-tjsf_2019.pdf a043e96c34c674727019fe478161aa71 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5045/5/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 5 TEXT Benito-tjsf_2019.pdf.txt Benito-tjsf_2019.pdf.txt Extracted text text/plain 65627 https://riubu.ubu.es/bitstream/10259/5045/6/Benito-tjsf_2019.pdf.txt 587b96a45ff34ce5041ab14bb17f5878 MD5 6 10259/5045 oai:riubu.ubu.es:10259/5045 2021-11-10 10:38:20.737 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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