2026-04-28T18:08:01Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/50762021-11-10T09:38:20Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Benito Román, Oscar Sanz Díez, Mª Teresa Melgosa Gómez, Rodrigo Paz Barragán, Esther de Escudero Barbero, Isabel Beltrán Calvo, Sagrario 2019-03-20T08:44:13Z 2019-05 2021-06 0896-8446 http://hdl.handle.net/10259/5076 10.1016/j.supflu.2018.07.026 Tyrosinase from mushroom was used as a model polyphenol oxidase (PPO) enzyme to perform a systematic inactivation study using High Pressure Carbon Dioxide (HPCD). The ratio CO2/volume of enzyme (g/mL) loaded in the reactor was found to be critical. Above a critical ratio, pressure, temperature and time did not control the inactivation performance. Exposure time (2–15 min), temperature (25–45 °C) and pressure (5–20 MPa) allowed to show a characteristic inactivation pattern for PPO: a sudden decrease in activity (at least 75% of the total activity loss was observed within the first 2 min) was followed by a slowed decay. The experimental data were fitted into a two fraction kinetic model and the main kinetic parameters (ZP, ZT activation volume and activation energy) were calculated. The fluorescence spectroscopy analysis of the samples treated with HPCD revealed significant changes in the tertiary structure of the enzyme. Spanish Government (MINECO) and the European Regional Development Fund (ERDF) for financial support of project CTQ2015-64396-R (MINECO/FEDER, UE). To Junta de Castilla y León and ERDF for O.Benito’s Post-doctoral contract funded by project BU055U16. To MINECO for E. de Paz’s Juan de la Cierva Contract (FJCI-2014-19850). application/pdf eng Elsevier The Journal of Supercritical Fluids. 2019, V. 147, p. 310-321 https://doi.org/10.1016/j.supflu.2018.07.026 info:eu-repo/grantAgreement/MINECO/CTQ2015-64396-R Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Polyphenol oxidase HPCD Enzyme inactivation Supercritical carbon dioxide Ingeniería química Chemical engineering Studies of polyphenol oxidase inactivation by means of high pressure carbon dioxide (HPCD) info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion