2026-04-28T15:17:27Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/50762021-11-10T09:38:20Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Benito Román, Oscar 59 500 0000-0002-4418-0045 Sanz Díez, Mª Teresa 530 500 Melgosa Gómez, Rodrigo 357 500 Paz Barragán, Esther de 421 500 Escudero Barbero, Isabel 161 500 Beltrán Calvo, Sagrario 57 500 2019-03-20T08:44:13Z 2019-05 2021-06 0896-8446 http://hdl.handle.net/10259/5076 10.1016/j.supflu.2018.07.026 Tyrosinase from mushroom was used as a model polyphenol oxidase (PPO) enzyme to perform a systematic inactivation study using High Pressure Carbon Dioxide (HPCD). The ratio CO2/volume of enzyme (g/mL) loaded in the reactor was found to be critical. Above a critical ratio, pressure, temperature and time did not control the inactivation performance. Exposure time (2–15 min), temperature (25–45 °C) and pressure (5–20 MPa) allowed to show a characteristic inactivation pattern for PPO: a sudden decrease in activity (at least 75% of the total activity loss was observed within the first 2 min) was followed by a slowed decay. The experimental data were fitted into a two fraction kinetic model and the main kinetic parameters (ZP, ZT activation volume and activation energy) were calculated. The fluorescence spectroscopy analysis of the samples treated with HPCD revealed significant changes in the tertiary structure of the enzyme. Spanish Government (MINECO) and the European Regional Development Fund (ERDF) for financial support of project CTQ2015-64396-R (MINECO/FEDER, UE). To Junta de Castilla y León and ERDF for O.Benito’s Post-doctoral contract funded by project BU055U16. To MINECO for E. de Paz’s Juan de la Cierva Contract (FJCI-2014-19850). application/pdf eng Elsevier The Journal of Supercritical Fluids. 2019, V. 147, p. 310-321 https://doi.org/10.1016/j.supflu.2018.07.026 info:eu-repo/grantAgreement/MINECO/CTQ2015-64396-R Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Polyphenol oxidase HPCD Enzyme inactivation Supercritical carbon dioxide Ingeniería química Chemical engineering Studies of polyphenol oxidase inactivation by means of high pressure carbon dioxide (HPCD) info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/5076/8/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 8 THUMBNAIL Benito-tjosf_2019.pdf.jpg Benito-tjosf_2019.pdf.jpg IM Thumbnail image/jpeg 3846 https://riubu.ubu.es/bitstream/10259/5076/7/Benito-tjosf_2019.pdf.jpg bfbb74de152f717a35ac116b018689a1 MD5 7 ORIGINAL Benito-tjosf_2019.pdf Benito-tjosf_2019.pdf application/pdf 1889662 https://riubu.ubu.es/bitstream/10259/5076/1/Benito-tjosf_2019.pdf b04b71648713004c41e8b15a2148e603 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5076/5/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 5 TEXT Benito-tjosf_2019.pdf.txt Benito-tjosf_2019.pdf.txt Extracted text text/plain 71487 https://riubu.ubu.es/bitstream/10259/5076/6/Benito-tjosf_2019.pdf.txt 69323e9cc4cb66000cbd4a135566869b MD5 6 10259/5076 oai:riubu.ubu.es:10259/5076 2021-11-10 10:38:20.77 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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