2026-06-30T05:19:14Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/51942022-02-28T23:42:08Zcom_10259_4365com_10259_5086com_10259_2604col_10259_4366

Rubio Antolin, Ana Rosa Fidalgo Zorrilla, Jairo Martin Vargas, Judit Pérez Arnáiz, Cristina Alonso de la Torre, Sara Biver, Tarita Espino Ordóñez, Gustavo Busto Vázquez, Natalia García Ruiz, Begoña 2019-12-16T10:32:02Z 2019-12-16T10:32:02Z 2020-02 0162-0134 http://hdl.handle.net/10259/5194 10.1016/j.jinorgbio.2019.110885 The synthesized 2-(hydroxy-1-naphtyl)imidazo-[4,5-f][1,10]phenanthroline (HNAIP) ligand and its new iridium ([Ir(ppy)2(HNAIP)]Cl) and rhodium ([Rh(ppy)2(HNAIP)]Cl) complexes, being ppy = 2-phenylpiridinate, show cytotoxic effects in SW480 (colon adenocarcinoma) and A549 (epithelial lung adenocarcinoma) cells. They all are cytotoxic in the tested cell lines. HNAIP and [Rh(ppy)2(HNAIP)]+ are the most cytotoxic, whereas [Ir(ppy)2(HNAIP)]+ displays negligible cytotoxicity towards A549 cells and moderate activity towards SW480. The interaction of all three compounds with Bovine Serum Albumin (BSA), l-glutathione reduced (GSH), nicotinamide adenine dinucleotide (NADH) and DNA was studied to explain the differences found in terms of cytotoxicity. None of them are able to interact with BSA, thus excluding bioavailability due to plasma protein interaction as the possible differentiating factor in their biological activity. By contrast, small differences have been observed regarding DNA interaction. In addition, taking advantage of the emission properties of these molecules, they have been visualized in the cytoplasmic region of A549 cells. Inductively coupled plasma mass spectrometry (ICP-MS) experiments show, in turn, that the internalization ability follow the sequence [Rh(ppy)2(HNAIP)]+ > [Ir(ppy)2(HNAIP)]+ > cisplatin. Therefore, it seems clear that the cellular uptake by tumour cells is the key factor affecting the different cytotoxicity of the metal complexes and that this cellular uptake is influenced by the hydrophobicity of the studied complexes. On the other hand, preliminary catalytic experiments performed on the photo-oxidation of GSH and some amino acids such as l-methionine (Met), l-cysteine (Cys) and l-tryptophan (Trp) provide evidence for the photocatalytic activity of the Ir(III) complex in this type of reactions. “la Caixa” Banking Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia, Innovación y Universidades (RTI2018-102040-B-100 and RTI2018-100709-B-C21), Junta de Castilla y León (BU305P18, FEDER Funds) application/pdf eng Elsevier Journal of Inorganic Biochemistry. 2019, V. 203, 110885 https://doi.org/10.1016/j.jinorgbio.2019.110885 info:eu-repo/grantAgreement/MICINN/RTI2018-102040-B-100 info:eu-repo/grantAgreement/MICINN/RTI2018-100709-B-C21 info:eu-repo/grantAgreement/JCyL/BU305P18 info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF/PR/PR12/11070003 http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional info:eu-repo/semantics/openAccess 2-(hydroxy-1-naphtyl)imidazo-[4,5-f] [1,10]phenanthroline Iridium Rhodium Cellular test DNA Photo-catalysis Bioquímica Química inorgánica Biochemistry Chemistry, Inorganic Biological activity and photocatalytic properties of a naphthyl-imidazo phenanthroline (HNAIP) ligand and its [Ir(ppy)2(HNAIP)]Cl and [Rh(ppy)2(HNAIP)]Cl complexes info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion