2026-05-14T14:56:55Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/51942022-02-28T23:42:08Zcom_10259_4365com_10259_5086com_10259_2604col_10259_4366

Rubio Antolin, Ana Rosa 491 500 Fidalgo Zorrilla, Jairo 723993db-eafe-481b-b4ee-71856c5e6268 500 Martin Vargas, Judit c32f0548-ba52-4a37-a111-e68423c7b368 500 Pérez Arnáiz, Cristina 435 500 Alonso de la Torre, Sara 20 500 Biver, Tarita 686e3c46-a69c-4fbe-9b7a-e05265bbac4e 500 Espino Ordóñez, Gustavo 162 500 Busto Vázquez, Natalia 83 500 García Ruiz, Begoña 217 500 2019-12-16T10:32:02Z 2019-12-16T10:32:02Z 2020-02 0162-0134 http://hdl.handle.net/10259/5194 10.1016/j.jinorgbio.2019.110885 The synthesized 2-(hydroxy-1-naphtyl)imidazo-[4,5-f][1,10]phenanthroline (HNAIP) ligand and its new iridium ([Ir(ppy)2(HNAIP)]Cl) and rhodium ([Rh(ppy)2(HNAIP)]Cl) complexes, being ppy = 2-phenylpiridinate, show cytotoxic effects in SW480 (colon adenocarcinoma) and A549 (epithelial lung adenocarcinoma) cells. They all are cytotoxic in the tested cell lines. HNAIP and [Rh(ppy)2(HNAIP)]+ are the most cytotoxic, whereas [Ir(ppy)2(HNAIP)]+ displays negligible cytotoxicity towards A549 cells and moderate activity towards SW480. The interaction of all three compounds with Bovine Serum Albumin (BSA), l-glutathione reduced (GSH), nicotinamide adenine dinucleotide (NADH) and DNA was studied to explain the differences found in terms of cytotoxicity. None of them are able to interact with BSA, thus excluding bioavailability due to plasma protein interaction as the possible differentiating factor in their biological activity. By contrast, small differences have been observed regarding DNA interaction. In addition, taking advantage of the emission properties of these molecules, they have been visualized in the cytoplasmic region of A549 cells. Inductively coupled plasma mass spectrometry (ICP-MS) experiments show, in turn, that the internalization ability follow the sequence [Rh(ppy)2(HNAIP)]+ > [Ir(ppy)2(HNAIP)]+ > cisplatin. Therefore, it seems clear that the cellular uptake by tumour cells is the key factor affecting the different cytotoxicity of the metal complexes and that this cellular uptake is influenced by the hydrophobicity of the studied complexes. On the other hand, preliminary catalytic experiments performed on the photo-oxidation of GSH and some amino acids such as l-methionine (Met), l-cysteine (Cys) and l-tryptophan (Trp) provide evidence for the photocatalytic activity of the Ir(III) complex in this type of reactions. “la Caixa” Banking Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia, Innovación y Universidades (RTI2018-102040-B-100 and RTI2018-100709-B-C21), Junta de Castilla y León (BU305P18, FEDER Funds) application/pdf eng Elsevier Journal of Inorganic Biochemistry. 2019, V. 203, 110885 https://doi.org/10.1016/j.jinorgbio.2019.110885 info:eu-repo/grantAgreement/MICINN/RTI2018-102040-B-100 info:eu-repo/grantAgreement/MICINN/RTI2018-100709-B-C21 info:eu-repo/grantAgreement/JCyL/BU305P18 info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF/PR/PR12/11070003 http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional info:eu-repo/semantics/openAccess 2-(hydroxy-1-naphtyl)imidazo-[4,5-f] [1,10]phenanthroline Iridium Rhodium Cellular test DNA Photo-catalysis Bioquímica Química inorgánica Biochemistry Chemistry, Inorganic Biological activity and photocatalytic properties of a naphthyl-imidazo phenanthroline (HNAIP) ligand and its [Ir(ppy)2(HNAIP)]Cl and [Rh(ppy)2(HNAIP)]Cl complexes info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion TEXT Rubio-jiib_2020.pdf.txt Rubio-jiib_2020.pdf.txt Extracted text text/plain 63850 https://riubu.ubu.es/bitstream/10259/5194/5/Rubio-jiib_2020.pdf.txt da46fd27a90da8c15ef5de638d4a44fe MD5 5 THUMBNAIL Rubio-jiib_2020.pdf.jpg Rubio-jiib_2020.pdf.jpg IM Thumbnail image/jpeg 4489 https://riubu.ubu.es/bitstream/10259/5194/4/Rubio-jiib_2020.pdf.jpg 3604bff424da89a79bfb072556ee53b2 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/5194/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/5194/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Rubio-jiib_2020.pdf Rubio-jiib_2020.pdf application/pdf 1599155 https://riubu.ubu.es/bitstream/10259/5194/1/Rubio-jiib_2020.pdf 59503b353ed004c8d57220143f3f6a1b MD5 1 10259/5194 oai:riubu.ubu.es:10259/5194 2022-03-01 00:42:08.32 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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