2026-04-28T04:13:42Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/52162024-07-24T11:45:35Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477

Domi, Brixhilda 791 500 0000-0001-7786-8615 Rumbo Lorenzo, Carlos 505 500 0000-0002-5038-0334 García Tojal, Javier 221 500 0000-0003-3816-0483 Elena Sima, Livia e16e1665-85bf-41f8-b203-057881eedf90 500 Negroiu, Gabriela ea8f5f36-24d8-407e-a0bf-e1c3ed366d6d 500 Tamayo Ramos, Juan Antonio 547 500 0000-0002-7071-002X 2020-02-19T09:41:56Z 2020-02-19T09:41:56Z 2020-01 http://hdl.handle.net/10259/5216 10.3390/ijms21010205 1422-0067 The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast Saccharomyces cerevisiae and the bacteria Vibrio fischeri to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria Lactobacillus plantarum and the AbG β-d-glucosidase from Agrobacterium sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, S. cerevisiae (colony forming units, ROS induction, genotoxicity) and V. fischeri (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial. European Union’s H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreements Nº 691095, Nº 721642 and Nº 734873; Junta de Castilla y Leon-FEDER under grants Nº BU079U16, BU291P18 and BU022G18, and Ministerio de Economía y Competitividad CTQ2016-75023-C2-1-P and CTQ2015-70371-REDT MetDrugs Network (Spain) application/pdf eng MDPI International Journal of Molecular Sciences. 2020, V. 21, n. 1, 205 https://doi.org/10.3390/ijms21010205 info:eu-repo/grantAgreement/EC/H2020/691095 info:eu-repo/grantAgreement/EC/H2020/721642 info:eu-repo/grantAgreement/EC/H2020/734873 info:eu-repo/grantAgreement/JCyL/BU079U16 info:eu-repo/grantAgreement/JCyL/BU291P18 info:eu-repo/grantAgreement/JCyL/BU022G18 info:eu-repo/grantAgreement/MINECO/CTQ2016-75023-C2-1-P info:eu-repo/grantAgreement/MINECO/CTQ2015-70371-REDT Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Graphene Unicellular organisms Toxicity Binding capacity ATR-FTIR TEM ICP-MS Materia-Composición Matter-Constitution Interaction analysis of commercial graphene oxide nanoparticles with unicellular systems and biomolecules info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion International Journal of Molecular Sciences 21 1 205 TEXT Domi-ijms_2020.pdf.txt Domi-ijms_2020.pdf.txt Extracted text text/plain 88630 https://riubu.ubu.es/bitstream/10259/5216/5/Domi-ijms_2020.pdf.txt 65690723bcd3553d71a4e6d2a57a171f MD5 5 THUMBNAIL Domi-ijms_2020.pdf.jpg Domi-ijms_2020.pdf.jpg IM Thumbnail image/jpeg 3344 https://riubu.ubu.es/bitstream/10259/5216/4/Domi-ijms_2020.pdf.jpg 7b7fc67bb3de0cf070a7d62fa2fd7582 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5216/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/5216/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Domi-ijms_2020.pdf Domi-ijms_2020.pdf application/pdf 5994290 https://riubu.ubu.es/bitstream/10259/5216/1/Domi-ijms_2020.pdf a943f4ed48f7582e2d94b0ca1dd24fdd MD5 1 10259/5216 oai:riubu.ubu.es:10259/5216 2024-07-24 13:45:35.634 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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