2026-05-07T05:38:22Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/52252024-07-24T11:46:02Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477

Suarez Diez, Maria 02e00910-18d5-46be-b42a-e43cca9f9803 500 Porras Alfonso, Santiago 667 500 0000-0003-4331-3085 Laguna Teno, Félix da8e7aaf-4c3d-47a4-8585-5bbe6587d5b2 500 Schaap, Peter J. f6d7edbb-1902-4228-b6fc-d769a702a41a 500 Tamayo Ramos, Juan Antonio 547 500 0000-0002-7071-002X 2020-03-02T09:19:39Z 2020-03-02T09:19:39Z 2020-02 http://hdl.handle.net/10259/5225 10.1038/s41598-020-60101-7 2045-2322 Graphene nanomaterials have attracted a great interest during the last years for different applications, but their possible impact on different biological systems remains unclear. Here, an assessment to understand the toxicity of commercial polycarboxylate functionalized graphene nanoplatelets (GN) on the unicellular fungal model Saccharomyces cerevisiae was performed. While cell proliferation was not negatively affected even in the presence of 800 mg L−1 of the nanomaterial for 24 hours, oxidative stress was induced at a lower concentration (160 mg L−1), after short exposure periods (2 and 4 hours). No DNA damage was observed under a comet assay analysis under the studied conditions. In addition, to pinpoint the molecular mechanisms behind the early oxidative damage induced by GN and to identify possible toxicity pathways, the transcriptome of S. cerevisiae exposed to 160 and 800 mg L−1 of GN was studied. Both GN concentrations induced expression changes in a common group of genes (337), many of them related to the fungal response to reduce the nanoparticles toxicity and to maintain cell homeostasis. Also, a high number of genes were only differentially expressed in the GN800 condition (3254), indicating that high GN concentrations can induce severe changes in the physiological state of the yeast. European Union’s H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreements N° 691095 and N° 734873; and Junta de Castilla y Leon-FEDER under grants N° BU079U16, and N° UBU-16-B. application/pdf eng Nature Research Scientific Reports. 2020, 10, art. 3232 https://doi.org/10.1038/s41598-020-60101-7 info:eu-repo/grantAgreement/EC/H2020/691095 info:eu-repo/grantAgreement/EC/H2020/734873 info:eu-repo/grantAgreement/JCyL/BU079U16 info:eu-repo/grantAgreement/JCyL/UBU-16-B Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Materiales Materials Toxicological response of the model fungus Saccharomyces cerevisiae to different concentrations of commercial graphene nanoplatelets info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Scientific Reports 10 1 TEXT Suarez-sc_20202.pdf.txt Suarez-sc_20202.pdf.txt Extracted text text/plain 63274 https://riubu.ubu.es/bitstream/10259/5225/5/Suarez-sc_20202.pdf.txt fe65e9f52d49f49e1a67bd2174a76165 MD5 5 THUMBNAIL Suarez-sc_20202.pdf.jpg Suarez-sc_20202.pdf.jpg IM Thumbnail image/jpeg 4790 https://riubu.ubu.es/bitstream/10259/5225/4/Suarez-sc_20202.pdf.jpg 7bd133f6200802605801b19d462d3d57 MD5 4 Suarez-sc_2020.pdf.jpg Suarez-sc_2020.pdf.jpg IM Thumbnail image/jpeg 4790 https://riubu.ubu.es/bitstream/10259/5225/6/Suarez-sc_2020.pdf.jpg 7bd133f6200802605801b19d462d3d57 MD5 6 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5225/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/5225/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Suarez-sc_2020.pdf Suarez-sc_2020.pdf application/pdf 1077586 https://riubu.ubu.es/bitstream/10259/5225/1/Suarez-sc_2020.pdf 0fa6ea094b10d28e0652bd88c070e14a MD5 1 10259/5225 oai:riubu.ubu.es:10259/5225 2024-07-24 13:46:02.639 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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