2026-05-05T03:12:02Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/53272022-04-14T22:42:06Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245
2020-06-05T11:09:59Z
urn:hdl:10259/5327
Polyphenol oxidase (PPO) and pectin methylesterase (PME) inactivation by high pressure carbon dioxide (HPCD) and its applicability to liquid and solid natural products
Benito Román, Oscar
Sanz Díez, Mª Teresa
Illera Gigante, Alba Ester
Melgosa Gómez, Rodrigo
Beltrán Calvo, Sagrario
PPO
PME
Juice
Supercritical carbon dioxide
Green process
Food technology
In the present work, High Pressure Carbon Dioxide (HPCD) has been used to inactivate the enzymes Polyphenol Oxidase (PPO) and Pectin Methylesterase (PME). The work has been done in two stages: in the first one, fundamental knowledge about the inactivation mechanism induced by HPCD was obtained using pure commercial enzymes. The influence of the main process parameters was studied: pressure, temperature, time and ratio CO2/volume of enzymatic solution. The changes in the tertiary structure of the enzymes after the HPCD treatment were analyzed by fluorescence spectroscopy running two different tests: intrinsic fluorescence measurement and ANS binding. In the second stage, the acquired knowledge was used to study the inactivation pattern of these two enzymes in liquid and solid natural products, including fruit and vegetable juices or shrimps. The results revealed differences in the inactivation pattern of the enzymes in these substrates compared to the pure commercial enzymes, but the applicability of HPCD technology for the inactivation of enzymes in real substrates at industrial scale was also revealed.
2020-06-05T11:09:59Z
2020-06-05T11:09:59Z
2020-04
info:eu-repo/semantics/article
0920-5861
http://hdl.handle.net/10259/5327
10.1016/j.cattod.2018.12.051
eng
Catalysis Today. 2020, V. 346, p. 112-120
https://doi.org/10.1016/j.cattod.2018.12.051
info:eu-repo/grantAgreement/JCyL/BU055U16
info:eu-repo/grantAgreement/MINECO/CTQ2015-64396-R
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Elsevier