2026-05-05T03:12:48Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53272022-04-14T22:42:06Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245

Benito Román, Oscar 59 500 0000-0002-4418-0045 Sanz Díez, Mª Teresa 530 500 Illera Gigante, Alba Ester 296 500 Melgosa Gómez, Rodrigo 357 500 Beltrán Calvo, Sagrario 57 500 2020-06-05T11:09:59Z 2020-06-05T11:09:59Z 2020-04 0920-5861 http://hdl.handle.net/10259/5327 10.1016/j.cattod.2018.12.051 In the present work, High Pressure Carbon Dioxide (HPCD) has been used to inactivate the enzymes Polyphenol Oxidase (PPO) and Pectin Methylesterase (PME). The work has been done in two stages: in the first one, fundamental knowledge about the inactivation mechanism induced by HPCD was obtained using pure commercial enzymes. The influence of the main process parameters was studied: pressure, temperature, time and ratio CO2/volume of enzymatic solution. The changes in the tertiary structure of the enzymes after the HPCD treatment were analyzed by fluorescence spectroscopy running two different tests: intrinsic fluorescence measurement and ANS binding. In the second stage, the acquired knowledge was used to study the inactivation pattern of these two enzymes in liquid and solid natural products, including fruit and vegetable juices or shrimps. The results revealed differences in the inactivation pattern of the enzymes in these substrates compared to the pure commercial enzymes, but the applicability of HPCD technology for the inactivation of enzymes in real substrates at industrial scale was also revealed. Spanish Government (MINECO) and the European Regional Development Fund (ERDF) for financial support of project CTQ2015-64396-R and A.E. Illera’s contract. To Junta de Castilla y León and ERDF for financial support of project BU055U16 and O. Benito-Román’s Post-doctoral contract. application/pdf eng Elsevier Catalysis Today. 2020, V. 346, p. 112-120 https://doi.org/10.1016/j.cattod.2018.12.051 info:eu-repo/grantAgreement/JCyL/BU055U16 info:eu-repo/grantAgreement/MINECO/CTQ2015-64396-R Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess PPO PME Juice Supercritical carbon dioxide Green process Food technology Ingeniería química Chemical engineering Polyphenol oxidase (PPO) and pectin methylesterase (PME) inactivation by high pressure carbon dioxide (HPCD) and its applicability to liquid and solid natural products info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Catalysis Today 346 112 120 TEXT Benito-ct_2020.pdf.txt Benito-ct_2020.pdf.txt Extracted text text/plain 58354 https://riubu.ubu.es/bitstream/10259/5327/5/Benito-ct_2020.pdf.txt aa1cd38e51e084a980cea8ef7ffbb920 MD5 5 THUMBNAIL Benito-ct_2020.pdf.jpg Benito-ct_2020.pdf.jpg IM Thumbnail image/jpeg 3669 https://riubu.ubu.es/bitstream/10259/5327/4/Benito-ct_2020.pdf.jpg 31861458c0791ca75ec367ae59a5270c MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5327/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/5327/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Benito-ct_2020.pdf Benito-ct_2020.pdf application/pdf 1194077 https://riubu.ubu.es/bitstream/10259/5327/1/Benito-ct_2020.pdf b2582a2e55f2409f9ba07f4129a10b4d MD5 1 10259/5327 oai:riubu.ubu.es:10259/5327 2022-04-15 00:42:06.389 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es RWwgYXV0b3IgY29tbyDDum5pY28gdGl0dWxhciBkZSBsb3MgZGVyZWNob3MgZGUgcHJvcGllZGFkIGludGVsZWN0dWFsIGRlIGxhIG9icmEsIG8gZGlzcG9uaWVuZG8gZGUgbG9zIGRlYmlkb3MgcGVybWlzb3MgZGUgbG9zIG90cm9zIHRpdHVsYXJlcywgc2kgbG9zIGh1YmllcmEsIHkgZW4gdmlydHVkIGRlIGxvcyBkZXJlY2hvcyBxdWUgbGUgY29uZmllcmUgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3IsIA0KQVVUT1JJWkEgYSBsYSBVbml2ZXJzaWRhZCBkZSBCdXJnb3MgYSBkaWZ1bmRpciwgZGUgbWFuZXJhIGdyYXR1aXRhLCBlbCBjb250ZW5pZG8gZGUgbG9zIGFyY2hpdm9zIGRpZ2l0YWxlcyBxdWUgY29ycmVzcG9uZGVuIGFsIGRvY3VtZW50byBkZXNjcml0byBhbnRlcmlvcm1lbnRlLCBjb24gY2Fyw6FjdGVyIG5vIGV4Y2x1c2l2byB5IGRlIG1hbmVyYSBww7pibGljYSBlbiBhY2Nlc28gYWJpZXJ0byBhIHRyYXbDqXMgZGUgSW50ZXJuZXQsIHBhcmEgbG8gcXVlIGxhIEJpYmxpb3RlY2EgcHJvY2VkZXLDoSBhIGFyY2hpdmFybG9zIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwuIEFzaW1pc21vIGF1dG9yaXphIGEgbGEgVW5pdmVyc2lkYWQgZGUgQnVyZ29zIGEgcmVhbGl6YXIgbGFzIHRyYW5zZm9ybWFjaW9uZXMgbmVjZXNhcmlhcyBkZSBmb3JtYXRvLCBubyBkZSBjb250ZW5pZG8sIHBhcmEgZ2FyYW50aXphciBsYSBwcmVzZXJ2YWNpw7NuIHkgZWwgYWNjZXNvIGVuIGVsIGZ1dHVyby4NCg0KRWwgYXV0b3IgZGlzcG9uZSwgZW4gdG9kbyBjYXNvLCBkZWwgZGVyZWNobyBhIHJldm9jYXIgZXN0YSBhdXRvcml6YWNpw7NuLg0KDQpMYSBjZXNpw7NuIGRlIGRlcmVjaG9zIGRlIGVzdGEgb2JyYSBzZSBlbmN1ZW50cmEgc3VqZXRhIGEgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3I=