2026-06-30T04:10:00Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53482022-04-08T10:34:38Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377

Cunha Silva, Hugo Pires, Filipa Velez Cabral, A. Cristina Dias Arcos Martínez, Julia 2020-06-18T08:42:57Z 2020-06-18T08:42:57Z 2019-12 0927-7765 http://hdl.handle.net/10259/5348 10.1016/j.colsurfb.2019.110490 Lactate oxidase (LOx), recognized to selectively catalyze the lactate oxidation in complex matrices, has been highlighted as preferable biorecognition element for the development of lactate biosensors. In a previous work, we have demonstrated that LOx crosslinking on a modified screen-printed electrode results in a dual range lactate biosensor, with one of the analysis linear range (4 to 50 mM) compatible with lactate sweat levels. It was advanced that such behavior results from an atypical substrate inhibition process. To understand such inhibition phenomena, this work relies in the study of LOx structure when submitted to increased substrate concentrations. The results found by fluorescence spectroscopy and dynamic light scattering of LOx solutions, evidenced conformational changes of the enzyme, occurring in presence of inhibitory substrate concentrations. Therefore, the inhibition behavior found at the biosensor, is an outcome of LOx structural alterations as result of a pH-dependent mechanism promoted at high substrate concentrations. Spanish Ministry of Science and Innovation (MICINN), Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (FEDER) (TEC20013-40561-P and MUSSEL RTC-2015-4077-2). Hugo Cunha-Silva would like to acknowledge funding from the Spanish Ministry of Economy (BES-2014-068214) application/pdf eng Elsevier Colloids and Surfaces B: Biointerfaces. 2019, V. 184, 110490 https://doi.org/10.1016/j.colsurfb.2019.110490 info:eu-repo/grantAgreement/MINECO/MUSSEL RTC-2015-4077-2 info:eu-repo/grantAgreement/MICINN/TEC20013-40561-P Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Lactate oxidase inhibition Amperometric biosensor Lactic acid Fluorescence spectrometry Dynamic light scattering Química analítica Chemistry, Analytic Inhibited enzymatic reaction of crosslinked lactate oxidase through a pH-dependent mechanism info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Colloids and Surfaces B: Biointerfaces 184 110490