2026-04-28T18:38:21Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53702021-11-02T12:01:31Zcom_10259_3924com_10259_5086com_10259_2604col_10259_3925

Facilitated Anion Transport Induces Hyperpolarization of the Cell Membrane That Triggers Differentiation and Cell Death in Cancer Stem Cells Soto Cerrato, Vanessa Manuel Manresa, Pilar . Hernando Santa Cruz, Elsa Calabuig Fariñas, Silvia Martínez Romero, Alicia Fernández Dueñas, Víctor Sahlholm, Kristoffer Knöpfel, Thomas García Valverde, María Rodilla, Ananda M. Jantus Lewintre, Eloisa Farràs, Rosa Ciruela, Francisco Pérez Tomás, Ricardo Quesada Pato, Roberto Facilitated anion transport potentially represents a powerful tool to modulate various cellular functions. However, research into the biological effects of small molecule anionophores is still at an early stage. Here we have used two potent anionophore molecules inspired in the structure of marine metabolites tambjamines to gain insight into the effect induced by these compounds at the cellular level. We show how active anionophores, capable of facilitating the transmembrane transport of chloride and bicarbonate in model phospholipid liposomes, induce acidification of the cytosol and hyperpolarization of plasma cell membranes. We demonstrate how this combined effect can be used against cancer stem cells (CSCs). Hyperpolarization of cell membrane induces cell differentiation and loss of stemness of CSCs leading to effective elimination of this cancer cell subpopulation. 2020-07-10T09:36:52Z 2020-07-10T09:36:52Z 2020-07-10T09:36:52Z 2015-12 info:eu-repo/semantics/article 0002-7863 http://hdl.handle.net/10259/5370 10.1021/jacs.5b09970 1520-5126 eng Journal of the American Chemical Society. 2015, V. 137, n. 50, p. 15892-15898 https://doi.org/10.1021/jacs.5b09970 info:eu-repo/semantics/openAccess American Chemical Society