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oai:riubu.ubu.es:10259/53712022-12-20T10:33:39Zcom_10259_3924com_10259_5086com_10259_2604col_10259_3925

2020-07-10T11:23:39Z urn:hdl:10259/5371 Determination of Volatiles in Mouse Urine by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry Arnáiz, Esther Moreno Mediavilla, Daniel Quesada Pato, Roberto Experimental design Gas chromatography-mass spectrometry (GC-MS) Headspace-solid-phase micro-extraction (HS-SPME) Mouse urine Volatile organic compounds The extraction and determination of volatile compounds in mice urine were performed using headspace solid-phase microextraction and gas chromatography-mass spectrometry. In order to optimize the extraction conditions, experimental design was applied. A sample volume of 108 µl, a temperature of 148.6°C, and a time of 94 minutes were found to be the optimal conditions. Samples of male and female mouse urine were analyzed to determine volatile compound profiles. A total of 36 organic compounds including ketones, aldehydes, and terpenes were detected. The results revealed that compounds such as 2-isopropyl-4,5-dihydrothiazole, which is considered a male sexual pheromone, were only detected in male urine samples, whereas others like benzaldehyde were especially abundant in female mouse urine. A comparison of female samples corresponding to different stages of the estrous cycle was also performed. 2020-07-10T11:23:39Z 2020-07-10T11:23:39Z 2014-03 info:eu-repo/semantics/article 0003-2719 http://hdl.handle.net/10259/5371 10.1080/00032719.2013.853182 1532-236X spa Analytical Letters. 2014, V. 47, n. 5, p. 721-729 https://doi.org/10.1080/00032719.2013.853182 info:eu-repo/semantics/openAccess Taylor & Francis Group