2026-04-22T15:06:13Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53712022-12-20T10:33:39Zcom_10259_3924com_10259_5086com_10259_2604col_10259_3925

00925njm 22002777a 4500 dc Arnáiz, Esther author Moreno Mediavilla, Daniel author Quesada Pato, Roberto author 2014-03 The extraction and determination of volatile compounds in mice urine were performed using headspace solid-phase microextraction and gas chromatography-mass spectrometry. In order to optimize the extraction conditions, experimental design was applied. A sample volume of 108 µl, a temperature of 148.6°C, and a time of 94 minutes were found to be the optimal conditions. Samples of male and female mouse urine were analyzed to determine volatile compound profiles. A total of 36 organic compounds including ketones, aldehydes, and terpenes were detected. The results revealed that compounds such as 2-isopropyl-4,5-dihydrothiazole, which is considered a male sexual pheromone, were only detected in male urine samples, whereas others like benzaldehyde were especially abundant in female mouse urine. A comparison of female samples corresponding to different stages of the estrous cycle was also performed. 0003-2719 http://hdl.handle.net/10259/5371 10.1080/00032719.2013.853182 1532-236X Experimental design Gas chromatography-mass spectrometry (GC-MS) Headspace-solid-phase micro-extraction (HS-SPME) Mouse urine Volatile organic compounds Determination of Volatiles in Mouse Urine by Headspace Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry