2026-05-05T16:54:33Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/54752022-11-30T23:42:08Zcom_10259_4365com_10259_5086com_10259_2604col_10259_4366

Macii, Francesca 83aa6ac0-e1f1-4ef9-8d5f-6e700cc20253 500 Pérez Arnáiz, Cristina 435 500 0000-0002-1468-9860 Arrico, Lorenzo e418b024-e33d-40ab-9c66-943bd4fd0bb3 500 Busto Vázquez, Natalia 83 500 0000-0001-9637-1209 García Ruiz, Begoña 217 500 0000-0002-0817-1651 Biver, Tarita 686e3c46-a69c-4fbe-9b7a-e05265bbac4e 500 2020-09-22T11:01:45Z 2020-09-22T11:01:45Z 2020-11 0162-0134 http://hdl.handle.net/10259/5475 10.1016/j.jinorgbio.2020.111199 This work concerns an analysis of the binding mechanism of a copper phthalocyanine (Alcian Blue-tetrakis(methylpyridinium) chloride, ABTP) to natural calf thymus DNA, G-quadruplexes (G4) and synthetic RNA polynucleotides in the form of double polyriboadenylic·polyribouridylic acid (poly(A)·poly(U)) or triple strands polyriboadenylic·2polyribouridylic acid (poly(A)·2poly(U)). ABTP is a well know dye that might undergo novel applications, but its interaction with DNA is scarcely studied and we lack information on possible RNA or G4 binding. This might be related to system complexity due to the presence of supramolecular dye-dye aggregates. Despite this, we show here that apparent parameters can be calculated, which provide information on the binding mechanism. Absorbance titrations in the presence of biosubstrate excess, melting and circular dichroism experiments show that ABTP binds to both RNAs and DNA. External/groove binding is the main feature for RNAs, whereas partial intercalation is the major binging mode for DNA. ABTP externally binds to both hybrid, parallel and anti-parallel G4s but seem to show a slightly different binding mode and a preference for anti-parallel structures. The thermodynamic features of the different systems are also discussed in the frame of the enthalpy-entropy compensation phenomenon. la Caixa” Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia, Innovación y Universidades-FEDER (RTI2018- 102040-B-100) and Junta de Castilla y León-FEDER (BU305P18) application/pdf eng Elsevier Journal of Inorganic Biochemistry. 2020, V. 212, 111199 https://doi.org/10.1016/j.jinorgbio.2020.111199 info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF/PR/PR12/11070003 info:eu-repo/grantAgreement/MICINN/RTI2018- 102040-B-100 info:eu-repo/grantAgreement/JCyL/U305P18 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Groove binding Partial intercalation Helix conformation Enthalpy-entropy compensation Anti-parallel Hybrid Química inorgánica Bioquímica Chemistry, Inorganic Biochemistry Alcian blue pyridine variant interaction with DNA and RNA polynucleotides and G-quadruplexes: changes in the binding features for different biosubstrates info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Journal of Inorganic Biochemistry 212 111199 TEXT Macii-jib-2020.pdf.txt Macii-jib-2020.pdf.txt Extracted text text/plain 45559 https://riubu.ubu.es/bitstream/10259/5475/5/Macii-jib-2020.pdf.txt f4192314bd43b7217d7947b8858a639a MD5 5 THUMBNAIL Macii-jib-2020.pdf.jpg Macii-jib-2020.pdf.jpg IM Thumbnail image/jpeg 4475 https://riubu.ubu.es/bitstream/10259/5475/4/Macii-jib-2020.pdf.jpg dc006f17bca5ba17dc2adf0ef5af8cf9 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/5475/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/5475/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Macii-jib-2020.pdf Macii-jib-2020.pdf application/pdf 1393269 https://riubu.ubu.es/bitstream/10259/5475/1/Macii-jib-2020.pdf c74054d47abc686d9531c7243a5b9c09 MD5 1 10259/5475 oai:riubu.ubu.es:10259/5475 2022-12-01 00:42:08.054 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es RWwgYXV0b3IgY29tbyDDum5pY28gdGl0dWxhciBkZSBsb3MgZGVyZWNob3MgZGUgcHJvcGllZGFkIGludGVsZWN0dWFsIGRlIGxhIG9icmEsIG8gZGlzcG9uaWVuZG8gZGUgbG9zIGRlYmlkb3MgcGVybWlzb3MgZGUgbG9zIG90cm9zIHRpdHVsYXJlcywgc2kgbG9zIGh1YmllcmEsIHkgZW4gdmlydHVkIGRlIGxvcyBkZXJlY2hvcyBxdWUgbGUgY29uZmllcmUgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3IsIA0KQVVUT1JJWkEgYSBsYSBVbml2ZXJzaWRhZCBkZSBCdXJnb3MgYSBkaWZ1bmRpciwgZGUgbWFuZXJhIGdyYXR1aXRhLCBlbCBjb250ZW5pZG8gZGUgbG9zIGFyY2hpdm9zIGRpZ2l0YWxlcyBxdWUgY29ycmVzcG9uZGVuIGFsIGRvY3VtZW50byBkZXNjcml0byBhbnRlcmlvcm1lbnRlLCBjb24gY2Fyw6FjdGVyIG5vIGV4Y2x1c2l2byB5IGRlIG1hbmVyYSBww7pibGljYSBlbiBhY2Nlc28gYWJpZXJ0byBhIHRyYXbDqXMgZGUgSW50ZXJuZXQsIHBhcmEgbG8gcXVlIGxhIEJpYmxpb3RlY2EgcHJvY2VkZXLDoSBhIGFyY2hpdmFybG9zIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwuIEFzaW1pc21vIGF1dG9yaXphIGEgbGEgVW5pdmVyc2lkYWQgZGUgQnVyZ29zIGEgcmVhbGl6YXIgbGFzIHRyYW5zZm9ybWFjaW9uZXMgbmVjZXNhcmlhcyBkZSBmb3JtYXRvLCBubyBkZSBjb250ZW5pZG8sIHBhcmEgZ2FyYW50aXphciBsYSBwcmVzZXJ2YWNpw7NuIHkgZWwgYWNjZXNvIGVuIGVsIGZ1dHVyby4NCg0KRWwgYXV0b3IgZGlzcG9uZSwgZW4gdG9kbyBjYXNvLCBkZWwgZGVyZWNobyBhIHJldm9jYXIgZXN0YSBhdXRvcml6YWNpw7NuLg0KDQpMYSBjZXNpw7NuIGRlIGRlcmVjaG9zIGRlIGVzdGEgb2JyYSBzZSBlbmN1ZW50cmEgc3VqZXRhIGEgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3Iu